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Merck
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CGD1

细胞生长测定试剂盒,基于MTT法

别名:

Mitochondrial activity assay

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
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packaging

pkg of 1 kit

Quality Level

technique(s)

UV/Vis spectroscopy: suitable, protein quantification: suitable

Amax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

shipped in

dry ice

storage temp.

−20°C

General description

细胞生长测定试剂盒(基于MTT)设计用于以分光光度法测量细胞生长,作为活细胞中线粒体活性的函数。MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯基溴化四唑)系统是一种简单、准确、可重复性的方法,可通过线粒体脱氢酶活性测量活细胞活性。

Application

细胞生长测定试剂盒(基于MTT)已用于:
  • 测定成骨前细胞的细胞活力
  • 正常人前列腺间质和上皮细胞的MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯溴化四唑)检测
  • 测定原代宫颈上皮细胞的细胞活力

Packaging

每个试剂盒包括5瓶试剂,每瓶含1 mL 5 mg/ml MTT的RPMI溶液(无酚红)和50 mL MTT溶剂(0.1N盐酸的无水异丙醇溶液)。 优化后的各组分体积大小尤其适合多孔板细胞生长。 MTT溶液按总培养体积的10%添加,因此每个试剂盒的测试次数取决于培养容量和/或体积大小。


signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

target_organs

Central nervous system

存储类别

3 - Flammable liquids

flash_point_f

53.6 °F - closed cup

flash_point_c

12 °C - closed cup

wgk

WGK 3

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

用于维持高质量经验证的无污染细胞培养的质量控制指南。免费下载ECACC手册。

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

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