Quality Level
usage
sufficient for 100 tests
shelf life
Kit is stable for 24 months when unopened.
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
General description
细胞谷胱甘肽过氧化物酶活性检测试剂盒用于对组织提取物中的谷胱甘肽过氧化物酶进行定量检测。本试剂盒基于谷胱甘肽（GSH）氧化后可生成氧化型谷胱甘肽（GSSG）。利用谷胱甘肽过氧化物酶（GPx）将GSH氧化成GSSG，生成的GSSG在谷胱甘肽还原酶（GR）的催化下再次生成GSH以及NADP+NAPDH氧化为NADP+的过程伴随340 nm处吸收光的减少。此处吸收值减少与样本中谷胱甘肽过氧化酶活性呈正相关。
Application
适用于测定组织提取物中的谷胱甘肽过氧化物酶
Biochem/physiol Actions
谷胱甘肽过氧化物酶细胞活性测定试剂盒用于测定组织提取物中的谷胱甘肽过氧化物酶。该测定基于将谷胱甘肽（GSH）氧化为氧化型谷胱甘肽（GSSG）。这一反应由GPx催化，并用谷胱甘肽还原酶和NADPH将GSSG再循环回GSH。由于该酶是偶联反应的限速因子，因此在NADPH氧化成NADP的过程中，可通过测定340nm处NADPH吸光度的降低，表明谷胱甘肽过氧化物酶的活性。
Other Notes
本试剂盒通过了兔网织红细胞裂解液和谷胱甘肽过氧化物酶的测试。
仅试剂盒组分
产品编号
说明
- tert-Butyl hydroperoxide 1 mL
signalword
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
存储类别
5.2 - Organic peroxides and self-reacting hazardous materials
法规信息
常规特殊物品
危险化学品
此项目有
商品
氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
B L Gupta et al.
Biochemistry and molecular biology international, 46(6), 1145-1152 (1999-01-19)
Experimentally induced diabetic rats were treated separately with insulin and vanadate. The activities of hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6PDH) were increased in reticulocyte hemolysate isolated from the diabetic rats and were restored to normal levels by insulin. The restoration
I Mavelli et al.
European journal of biochemistry, 139(1), 13-18 (1984-02-15)
Red blood cells of favism patients with acute hemolytic crisis have markedly more superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) and less glutathione peroxidase (glutathione:hydrogenperoxide oxidoreductase, EC 1.11.1.9) than either normal controls, glucose-6-phosphate dehydrogenase-deficient subjects or favism patients outside hemolytic crisis.
Matthias L Jauslin et al.
Human molecular genetics, 11(24), 3055-3063 (2002-11-06)
Friedreich Ataxia (FRDA), the most prevalent of the inherited ataxias, is a multi-systemic disease with loss of sensory neurons and life-threatening hypertrophic cardiomyopathy as its most severe manifestations. Reduced levels of the mitochondrial protein frataxin lead to cell-damaging oxidative stress
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CGP1-1KT | 04061833531761 |