usage
sufficient for 100 tests
Quality Level
packaging
pkg of 1 kit
storage condition
dry at room temperature
detection method
colorimetric
shipped in
dry ice
storage temp.
−20°C
General description
衰老细胞组织化学染色试剂盒含有鉴定衰老细胞所需的所有试剂，测试基于pH 6下β-半乳糖苷酶活性的组织化学染色。β-衰老细胞中的半乳糖苷酶活性是可检测的，靜止細胞、永生细胞或肿瘤细胞则无法检测。细胞衰老是一系列事件的进展，细胞借此由活跃分裂转为不分裂期。细胞衰老过程和老化相关。细胞分裂的减少几乎是不可逆的、完全的。除了丧失分裂能力外，细胞的形态学、形状和物理外观，以及它们的基因表达模式也发生变化。在该过程的结束时，细胞常常死亡，尽管细胞可能仍长期保持活力。
Application
衰老细胞组织化学染色试剂盒已被用于：
- 与衰老相关的β-半乳糖苷酶测试
- 测试人胰腺癌细胞
- 脂肪源性干细胞（ADSC）
- 脐血间充质/干细胞（CBMSC）中的细胞衰老
Biochem/physiol Actions
复制性衰老是一种生长停滞状态，主要表现为细胞失去分裂潜能，同时细胞形态、形状、生理特征和基因表达谱发生变化。
Legal Information
本产品根据美国专利号：第5491069和5795728号授权生产。
仅试剂盒组分
产品编号
说明
- X-gal solution 4 mL
- Staining Solution, 10X 15 mL
- Fixation Buffer 10x 15 mL
- Reagent B 1.5 mL
- Reagent C 1.5 mL
- Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
supp_hazards
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
商品
基于细胞的细胞增殖（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，可应用于癌症、神经科学和干细胞研究。
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
G P Dimri et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(20), 9363-9367 (1995-09-26)
Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is
Michael J MacLeod et al.
Journal of fish diseases, 41(9), 1359-1372 (2018-06-09)
In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and
Mandy Oj Grootaert et al.
Autophagy, 11(11), 2014-2032 (2015-09-24)
Autophagy is triggered in vascular smooth muscle cells (VSMCs) of diseased arterial vessels. However, the role of VSMC autophagy in cardiovascular disease is poorly understood. Therefore, we investigated the effect of defective autophagy on VSMC survival and phenotype and its
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CS0030-1KT | 04061833553060 |