D0550
3,5-二硝基水杨酸
used in colorimetric determination of reducing sugars
别名:
2-羟基-3,5-二硝基苯甲酸, DNS
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关于此项目
线性分子式:
(O2N)2C6H2-2-(OH)CO2H
化学文摘社编号:
分子量:
228.12
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
210-204-3
Beilstein/REAXYS Number:
2220661
MDL number:
Assay:
≥98% (HPLC and titration)
Form:
powder
Quality Level
assay
≥98% (HPLC and titration)
form
powder
mp
168-172 °C (lit.)
solubility
H2O: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
InChI
1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
InChI key
LWFUFLREGJMOIZ-UHFFFAOYSA-N
Application
已经使用 3，5-二硝基水杨酸（DNS）来停止体外 α-淀粉酶抑制研究中的反应。它还用于酶促释放的还原糖的定量。
Biochem/physiol Actions
3，5-二硝基水杨酸（DNS）用于还原糖的比色测定，并通过定量酶释放的还原糖来分析糖苷酶（糖苷水解酶）活性。已经将二硝基水杨酸方法与 Nelson-Somogi 比色法进行了比较。
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1
存储类别
4.1A - Other explosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
实验方案
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities.
Gusakov, A.V., et al.
International Journal of Antimicrobial Agents, e283658-e283658 (2011)
Use of dinitrosalicylic acid reagent for determination of reducing sugar.
Miller, G.L.
Analytical Chemistry, 31, 426-428 (1959)
Reducing value methods for maltodextrins. I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper.
J F Robyt et al.
Analytical biochemistry, 45(2), 510-516 (1972-02-01)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D0550-500G | 04061835347377 |
| D0550-100G | 04061835347346 |
| D0550-10G | 04061835347353 |
| D0550-25G | 04061835347360 |