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Merck
CN

D0672

φX174 DNA Marker Hae III Digest

for DNA electrophoresis

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关于此项目

NACRES:
NA.25
UNSPSC Code:
41105335
Form:
liquid
Storage temp.:
−20°C
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Quality Level

form

liquid

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Preparation Note

φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.


存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.





全球贸易项目编号

货号GTIN
D0672-.1MG04061833557969
D0672-20UG04061835572465