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Merck
CN

D0942

Anti-DHFR, C-terminal antibody produced in rabbit

1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-Dihydrofolate reductase

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB CL
Citations:
4
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human, rat

concentration

1.0-1.5 mg/mL

technique(s)

immunoprecipitation (IP): 0.5-1.0 μg using 100-200 ng of purified DHFR, western blot (chemiluminescent): 0.5-1.0 μg/mL using 50-100 ng of purified recombinant DHFR, or 293T human embryonic kidney cell extract, or Jurkat human acute T Cell leukemia cell extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DHFR(1719)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)

Immunogen

amino acids 171-185 of mouse DHFR, conjugated to KLH via an N-terminal added cysteine residue.

Biochem/physiol Actions

DHFR and DHFR fusion proteins. The epitope resides within amino acids 171-185 of mouse DHFR.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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分析证书(COA)

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Soledad Chazarra et al.
Applied biochemistry and biotechnology, 162(7), 1834-1846 (2010-04-16)
Recent reports describe the inhibition of human dihydrofolate reductase (hDHFR) by natural tea polyphenols. This finding could explain the epidemiologic data on their prophylactic effects for certain forms of cancer, and it raises the possibility that natural and synthetic polyphenols
Gilad Barshad et al.
Scientific reports, 9(1), 9987-9987 (2019-07-12)
Mitochondrial complex I (CI) is the largest multi-subunit oxidative phosphorylation (OXPHOS) protein complex. Recent availability of a high-resolution human CI structure, and from two non-human mammals, enabled predicting the impact of mutations on interactions involving each of the 44 CI
Stephen W Michnick et al.
Methods in molecular biology (Clifton, N.J.), 756, 395-425 (2011-08-27)
Protein-fragment Complementation Assays (PCAs) are a family of assays for detecting protein-protein interactions (PPIs) that have been developed to provide simple and direct ways to study PPIs in any living cell, multicellular organism, or in vitro. PCAs can be used



全球贸易项目编号

货号GTIN
D0942-100UG04061833558232