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D4418

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

别名:

DAB, DAB tablets, Immunohistology substrate

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关于此项目

化学文摘社编号:
91-95-2
UNSPSC Code:
12352204
PubChem Substance ID:
24893872
NACRES:
NA.83
MDL number:
MFCD00007725
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产品名称

SIGMAFAST™ 3,3′-二氨基联苯胺片, tablet, To prepare 15 mL

Quality Level

200

form

tablet

solubility

water: 1 tablet/5 mL

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

Application

本品适于作为沉淀底物，在免疫组织和免疫印迹分析中原位检测过氧化物酶活性。DAB会生成高强度的耐酒精黑棕色色斑，是免疫组织分析的首选底物。经DAB染色后，玻片可进一步进行脱水和树脂封片处理，以便保存供后续比对。SIGMAFAST™ DAB片剂不需要另外的缓冲液或步骤来配制活性底物溶液。铝箔独立包装。

Preparation Note

将每片片剂溶于 15 mL 去离子水中，得到含 DAB 和过氧化氢尿素的即用型缓冲液。

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCD1661
SLBR2966V
SLBM5774V
SLBF1437
SLBH5441V

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实验方案

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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商品

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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全球贸易项目编号

货号GTIN
D4418-5SET04061835572663
D4418-50SET04061835572656