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D5030

杜氏改良 Eagle 培养基-低葡萄糖

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

别名:

DME, DMEM

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

最基本的配方。这个配方适用于希望从DMEM培养基的基础成分开始研究的实验人员，可以根据应用灵活优化配方。
杜氏改良Eagle培养基（DMEM）是对Eagle基础培养基 (BME)的改良，含有四倍浓度的氨基酸和维生素。它包括额外的补充部分。据报告首个DME配方用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。用4,500 mg/L葡萄糖修饰培养基对培养某些类型的细胞效果最佳。

Application

杜氏改良Eagle′培养基已用于：
  • 培养分离的指短屈肌（FDB）纤维，以测量外源脂肪酸（FA）的利用率，作为耗氧率（OCR）测量的一部分
  • 培养离体胫骨前肌纤维以测定乳酸含量
  • 培养用于成骨分化的人骨髓间充质干细胞，制备培养人肝细胞癌HepG2细胞的低糖培养基

Preparation Note

按照每升培养基8.3克粉末配制。
补充1.0g/L葡萄糖，0.584g/L L-谷氨酰胺，3.7g/L碳酸氢钠。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCL8142
SLCG2659
SLBW2010
SLBW1406
SLBS0115V

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实验方案

Cultrex®基底膜提取物，Type 2，PathClear®实验方案

Cultrex®基底膜提取物（BME）是从Engelbreth-Holm-Swarm (EHS) 肉瘤细胞中纯化出来的一种可溶性基底膜。

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

Cultrex® 3-D Culture Matrix™ Laminin I Protocol

3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.

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商品

钙在细胞培养中的应用

钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

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Dulbecco Modified Eagle Medium (DME)

Instructions

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货号GTIN
D5030-50K04061834180272
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D5030-10X1L04061833585979
D5030-50L04061833585986