D6883
2′,7′-二氯荧光素二乙酸酯
≥97% (HPLC), powder, cell-permeable non-fluorescent probe
别名:
DCFH-DA, 2′,7′-二氯二氢荧光素二乙酸酯
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关于此项目
经验公式（希尔记法）:
C24H16Cl2O7
化学文摘社编号:
分子量:
487.29
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
Beilstein/REAXYS Number:
4610273
产品名称
2′,7′-二氯荧光素二乙酸酯, ≥97%
Quality Level
assay
≥97%
form
powder
storage temp.
−20°C
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI key
PXEZTIWVRVSYOK-UHFFFAOYSA-N
General description
2′,7′-二氯二氢荧光素二乙酸酯（DCFH-DA）是一种对氧化敏感的荧光染料，用于检测细胞内活性氧（ROS）水平。DCFH-DA是一种细胞渗透性非荧光探针，可被细胞内酯酶水解为DCFH，当被细胞内的ROS氧化时形成荧光DCF。
激发波长是504 nm，发射波长是529 nm（溶于乙醇）。尚未测试过DMSO溶剂，不过已有多篇文献表明这种溶剂适合使用。
Application
2′,7′-二氯荧光素二乙酸酯适用于：
- 敏感并快速定量活性氧，以响应氧化代谢
- 微孔板法，以检测吞噬细胞中的氧化产物
- 定量多孔髓样分化测定
适用于：敏感并快速定量活性氧，以响应氧化代谢；微孔板法，以检测吞噬细胞中的氧化产物；髓样分化定量多孔测定。
Preparation Note
本品应在-20℃下以粉末形式保存。
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
商品
氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D6883-50MG | 04061835361229 |
| D6883-250MG | 04061838445964 |