Quality Level
form
liquid
usage
100 uses
solubility
water: soluble
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma的DirectLoad宽分子量 DNA 分子量标准包含16个从50 - 10,000 bp并带有规则间隔的片段。10 ul分子量标准可直接上样至琼脂糖或聚丙烯酰胺凝胶上的单个泳道中。
Application
适于通过琼脂糖或聚丙烯酰胺凝胶测定PCR产生的DNA片段的大小。
Features and Benefits
- 可直接上样
- 易于使用
- 常见的条带大小
Other Notes
Sigma的DirectLoad™宽分子量 DNA 分子量标准以含有2.5% Ficoll(400型)、0.0125%溴酚蓝以及0.00625%二甲苯蓝的凝胶上样溶液形式提供。
为实现最佳分辨率,建议的琼脂糖凝胶浓度为0.75 - 2.0%。
含有16个50 - 10,000 bp片段,在琼脂糖凝胶上呈现合理间隔的条带。
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
实验方案
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
商品
选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
相关内容
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
Melanie P Dautle et al.
Journal of clinical microbiology, 40(2), 414-421 (2002-02-05)
In this study, 83 clinical isolates purified from biofilms colonizing 18 silicone gastrostomy devices (12 "buttons" and six tubes converted to skin level devices) were selected for subtype characterization utilizing genetic analysis. The tubes, previously used for feeding, remained in
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D7058-1VL | 04061835572724 |