D7295
脱氧核苷酸混合物，10mM
Molecular Biology Reagent
别名:
脱氧核苷酸混合物，10mM
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
NACRES:
NA.52
UNSPSC Code:
41106305
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
liquid
Solubility:
H2O: soluble at 20 °C
Storage temp.:
−20°C
biological source
synthetic (organic)
Quality Level
assay
≥99%
form
liquid
concentration
10 mM
color
colorless
solubility
H2O: soluble at 20 °C
application(s)
agriculture
foreign activity
DNase, RNase, none detected
shipped in
dry ice
storage temp.
−20°C
General description
核苷酸是有机分子，可作为核酸（如DNA和RNA）的单体或亚基。核苷酸是核酸的结构单元，由一个含氮碱基（嘌呤或嘧啶）、一个五碳糖（核糖或脱氧核糖）和至少一个磷酸基团组成。一个核苷与一个磷酸基团组合形成一个核苷酸。脱氧核苷酸混合物是一种便捷式预混dNTP溶液，以高质量分子生物学级水为溶剂，含有等摩尔（10mM）的UltraPure超纯dATP、dCTP、dGTP和TTP钠盐。1 µL足够进行一次标准50 µL PCR反应。
Application
dNTP混合物已用于：
- PCR扩增昆虫、 真菌、病毒、人类来源分离的基因组DNA
- 总RNA逆转录为cDNA
- 作为聚合酶链式反应(PCR)DNA扩增混合物组分
- 常规和长片段PCR
- 手动和自动DNA测序
- cDNA合成和标记反应
Features and Benefits
- 每种dNTP的纯度：最低99%
- 方便配制；每50 μL PCR使用1 μL
- 等摩尔量的各种dNTP可减少移液步骤
- 最大限度地降低PCR污染风险
- UltraPure超纯dNTP可帮助最大限度地提高关键PCR反应的一致性和得率
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
了解标准 PCR 方案步骤，查看试剂清单或循环参数。本方法使用标准 Taq DNA 聚合酶对 DNA 进行常规 PCR 扩增。
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 8-8 (1989)
Fiona McDonnell et al.
PloS one, 11(4), e0153354-e0153354 (2016-04-29)
Fibrosis and a hypoxic environment are associated with the trabecular meshwork (TM) region in the blinding disease glaucoma. Hypoxia has been shown to alter DNA methylation, an epigenetic mechanism involved in regulating gene expression such as the pro-fibrotic transforming growth
Shangmei Cao et al.
Journal of ethnopharmacology, 244, 112104-112104 (2019-08-09)
ShenYanXiaoBai granules is a traditional Chinese herbal medicine, It is used widely for the treatment of proteinuria caused by various kidney diseases. This study investigated the mechanism of Shenyan Xiaobai Granule in the treatment of nephritis proteinuria. 100 male wistar
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D7295-.5ML | 04061838104946 |
| D7295-5ML | 04061838104953 |
| D7295-.25ML | 04061838056863 |
| D7295-.2ML | 04061838104939 |
| D7295-20X.2ML | 04061838087355 |