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Merck
CN

D7777

杜氏改良 Eagle 培养基 - 高葡萄糖

With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

别名:

DME, DMEM

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
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Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。

Preparation Note

按照每升培养基13.5克粉末配制。
补充 3.7g/L 碳酸氢钠


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存储类别

13 - Non Combustible Solids

wgk

WGK 1



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

粉末培养基及盐混合物极具吸湿性,应注意防潮。

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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