产品名称
杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L
shipped in
ambient
storage temp.
2-8°C
General description
在成分确定的培养基研究中，通常用成分确定的混合物（包括营养素、生长因子和激素）替代血清添加到培养基中。常用的组合是Dulbecco′s改良Eagle培养基(DME)与Ham′s F-12营养混合物（1:1混合）。为了补偿去血清后的缓冲能力损失，可在配方中添加15 mM终浓度的HEPES缓冲液，帮助研究人员优化缓冲体系。
Application
Dulbecco′s改良Eagle培养基/Ham F-12营养混合物已用于：
- 维持人肺和结直肠癌细胞系生存，进行次级代谢产物的生物学评估
- 培养人肾-2细胞(HK-2)并作为检测培养基组分，用于溶血磷脂酰胆碱(LPC)处理
- 小鼠鳞状细胞癌(SCCVII)肿瘤细胞培养
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Nutrient Mixture F-12 Ham Formulation
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Silver nanoparticles (AgNP) emerged as a promising reagent for cancer therapy with oxidative stress implicated in the toxicity. Meanwhile, studies reported cold atmospheric plasma (CAP) generation of reactive oxygen and nitrogen species has selectivity towards cancer cells. Gold nanoparticles display
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D8062-6X500ML | 04061833589182 |
| D8062-500ML | 04061833589175 |