biological source
bovine (calf) thymus
Quality Level
form
lyophilized powder
extent of labeling
≥3 mg single-stranded calf thymus DNA/g
storage temp.
−20°C
General description
小牛胸腺 DNA 单链脱氧核糖核酸−纤维素又称ctDNA,是一种用于 DNA 结合剂研究的天然 DNA 来源。 ctDNA 的检测限约为 2.0ng,光散射强度(I(LS))的线性范围为 0.05-1.5mµg。
Application
小牛胸腺 DNA 脱氧核糖核酸−纤维素单链用于研究多种蛋白质、核苷酸和化学试剂(包括抗癌药物)的结合参数和结合特异性。 它常用于pull-down分析和色谱程序。
Analysis Note
可能含有微量的 Tris 和 EDTA。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
A Georgaki et al.
Nucleic acids research, 22(7), 1128-1134 (1994-04-11)
A DNA helicase from calf thymus, called DNA helicase F, copurified with replication protein A through several steps of purification including DEAE-Sephacel, hydroxyapatite and single stranded DNA cellulose. It is finally separated from replication protein A on FPLC Mono Q
S S Zhang et al.
The Journal of biological chemistry, 266(30), 20483-20490 (1991-10-25)
Two DNA helicases from calf thymus nuclei have been purified and characterized. The two proteins, designated as nuclear DNA helicase I and II, were copurified on Bio-Rex 70, DEAE-Sepharose, phosphocellulose and subsequently separated from each other on a heparin-Sepharose column.
T Kuzuhara et al.
Biochemical and biophysical research communications, 362(4), 805-810 (2007-09-04)
Tumor necrosis factor-alpha (TNF-alpha) inducing protein (Tipalpha) is a carcinogenic factor secreted from Helicobacter pylori (H. pylori), mediated through both enhanced expression of TNF-alpha and chemokine genes and activation of nuclear factor-kappaB. Since Tipalpha enters gastric cancer cells, the Tipalpha
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D8273-5G | 04061833662755 |
| D8273-10G | 04061833589342 |
| D8273-1G | 04061826690659 |