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D9154

邻联茴香胺 二盐酸盐

tablet

别名:

3,3'-二甲氧基联苯胺 二盐酸盐, 固蓝 B

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关于此项目

经验公式（希尔记法）:
C14H16N2O2 · 2HCl
化学文摘社编号:
20325-40-0
分子量:
317.21
NACRES:
NA.83
PubChem Substance ID:
24894263
UNSPSC Code:
12352204
EC Number:
243-737-5
MDL number:
MFCD00012488
Beilstein/REAXYS Number:
3917996
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产品名称

邻联茴香胺 二盐酸盐, tablet, 10 mg substrate per tablet

Quality Level

200

form

tablet

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

Biochem/physiol Actions

邻联茴香胺（3,3′-dimethoxybenzidine）是一种适合在 ELISA 步骤中使用的过氧化物酶底物。该底物产生可溶性终产物，颜色为黄橙色，可在 405 nm 处用分光光度法读取。可用 5 MHCl 终止反应。

Preparation Note

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

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可能致癌。

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

法规信息

危险化学品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCD6994
SLBX3894
SLBR5244V
SLBN0046V
SLBL2762V

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实验方案

胆固醇氧化酶的酶学测定

本操作流程使用过氧化物酶和邻联二茴香胺在500nm处进行胆固醇氧化酶活性的测定。胆固醇氧化酶是一种以氧为受体而作用于供体的CH-OH基团的酶。

Enzymatic Assay of Cholesterol Oxidase

Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

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比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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全球贸易项目编号

货号GTIN
D9154-100TAB04061833590317
D9154-50TAB04061833590324