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Merck
CN

D9785

DMEM/F-12

With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure

别名:

杜氏改良Eagle 培养基/营养混合物 F-12 Ham, DME/F-12, 1:1混合

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
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产品名称

DMEM/营养混合物 F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO3: no
phenol red: no
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

General description

提供的最基本的制剂。该制剂由希望从 (1;1) DME/Ham F-12 的必需组分开始,并具有为自身应用优化制剂的灵活性的研究者使用。该基础制剂特别适用于标记氨基酸的细胞蛋白代谢研究。

Preparation Note

补充 0.1545 g/L 氯化钙,0.365 g/L L -谷氨酰胺,0.05905 g/L L -亮氨酸,0.09125 g/L L -赖氨酸,0.0612 g/L 氯化镁,0.04884 g/L 硫酸镁,0.0172 g/L L -蛋氨酸,1.2 g/L 碳酸氢钠。
配制成每升培养基含 14.8 克粉末。


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存储类别

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

粉末培养基及盐混合物极具吸湿性,应注意防潮。

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

商品

钙是一种离子稳定的二价阳离子,在细胞培养基中具有有益和有毒的双重属性,可作为一系列细胞培养基的组成部分。

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.


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全球贸易项目编号

货号GTIN
D9785-50KG04061834408475
D9785-25KG04061834408468
D9785-10L04061833591215