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E1014

Benzonase®核酸酶

≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

别名:

内切核酸酶 来源于粘质沙雷氏菌

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关于此项目

化学文摘社编号:
9025-65-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC 号:
3.1.30.2 (BRENDA, IUBMB)
MDL number:
MFCD00131010
Assay:
≥90% (SDS-PAGE)
Biological source:
Serratia marcescens
Recombinant:
expressed in E. coli
Concentration:
≥250 units/μL
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biological source

Serratia marcescens

Quality Level

200

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

30 kDa

concentration

≥250 units/μL

application(s)

research use

foreign activity

protease, essentially free

shipped in

wet ice

storage temp.

−20°C

General description

Benzonase®全能核酸酶是一种通过高效基因工程法从粘质沙雷氏菌(Serratia marcescens)中获得的核酸内切酶。这种由两个必需二硫键连接的二聚体蛋白可在宽泛的操作条件下攻击并降解所有形式的DNA和RNA（单链、双链、线性和环化）。Benzonase®全能核酸酶能够去除核酸并提高蛋白质样本的纯度和质量。

Application

Benzonase®全能核酸酶用于：作为冰冷裂解缓冲液C的组分以消化DNA 和  RNA，便于充分释放所有核蛋白在免疫沉淀中用于释放核质和染色质中的蛋白复合物作为RIPA添加剂，用于分离SHSY5Y细胞进行免疫沉淀在脱细胞方法中用于去除主动脉根部的残留核酸
用于去除蛋白样品中的核酸。

Biochem/physiol Actions

Benzonase®全能核酸酶能将核酸完全消化成长度为3到5个碱基的5′-单磷酸末端寡核苷酸，使其成为从重组蛋白中去除核酸的理想工具，并适于需要完全消化核酸的应用。除了降低蛋白质提取物的粘度并防止细胞结团之外，使用Benzonase®全能核酸酶预处理蛋白质样本可以消除任何结合的核酸，进而显著提高2D凝胶电泳的分辨率。这种多功能酶可消化天然或热变性的DNA和RNA，其酶活性的最佳pH值为8.0-9.2。Benzonase®全能核酸酶可有效去除微生物组样本中的宿主DNA。在许多情况下，微生物组样本（如唾液或皮肤等）中含有较高比例的能够干扰下游结果的宿主DNA。我们的专家表示，减少宿主DNA可以降低测序成本，同时增加并改进数据。实验数据显示于以下技术文章中-用于微生物组工作流程的Benzonase®全能核酸酶物
消化天然或热变性的DNA和RNA。

Features and Benefits

  • 去除微生物组样本的宿主DNA。


  • 在各种工作流程中高效去除核酸。


  • 在蛋白提取过程中降低粘度。

Physical form

在含有20 mM Tris HCl, pH 8.0, 2 mM MgCl2及20 mM NaCl的50%甘油中的溶液。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
在pH 8.0，37 °C条件下，一个单位的酶在30分钟内可将超声的鲑鱼精子DNA消化成等同于1.0 ΔA260的酸可溶寡核苷酸（2.625 ml反应体积）。

Legal Information

Benzonase®核酸酶由德国达姆施塔特默克公司和/或其附属公司提供。
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCL6917
SLCL2845
SLCK8761
SLCK9347
SLCL1729

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Benzonase® 核酸酶常见问题解答

本页列出了有关 Benzonase® 核酸酶的九个常见问题及其解答。

Benzonase® Nuclease Q&A

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

Benzonase® Nuclease Q&A

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

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全球贸易项目编号

货号GTIN
E1014-25KU04061835572915
E1014-5KU04061835572922