E6779
EZview™ Red 抗-HA 亲和凝胶
别名:
抗-HA
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
NACRES:
NA.56
UNSPSC Code:
12352203
Technique(s):
affinity chromatography: suitable, immunoprecipitation (IP): suitable
Species reactivity:
-
Application:
IP, affinity chromatography
Citations:
105
shelf life
1 yr at 2‑8 °C
Quality Level
analyte chemical class(es)
proteins (hemagglutinin)
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
matrix
crosslinked agarose
capacity
≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)
storage temp.
2-8°C
General description
EZview Red 抗-HA 亲和凝胶是一种红色的抗HA琼脂糖亲和凝胶，含有共价连接在交联琼脂糖珠上的抗HA单克隆抗体。HA标签含有YPYDVPDYA序列，来源于血凝素（HA）蛋白。该序列位于全长 HA 蛋白的氨基酸残基 98-106 处。HA 标签存在大量带电氨基酸，这增强了其强抗体识别的可能性。
Application
EZview™ Red Anti-HA亲和凝胶已用于免疫印迹分析和免疫沉淀测定。
当进行小规模的亲和捕获时，如免疫沉淀，在微离心管中很难看到亲和基质。 偶然吸入树脂会导致定量结果变化。The EZview™ Red 亲和凝胶大大降低了颗粒损失的风险。 EZview树脂的性能和传统的非彩色亲和凝胶一样好，然而它使得用户很容易地从上清中辨别颗粒。 这与更准确的数据关联，因为蛋白质损失更少。
适用于亲和捕获（免疫沉淀） 大肠杆菌 或哺乳动物细胞中N-端或C-端带HA标记融合蛋白。
Biochem/physiol Actions
结合特异性：HA-标签多肽(N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C)。它可以识别HA标签蛋白的还原形式，并与N-端或C-端HA标签融合蛋白反应。
Features and Benefits
- 增加可视性-红色减少了偶然吸的风险
- 通过降低偶然损失而提高目的蛋白的回收率
- 更高的重现性-更一致的结果
Physical form
1:1 (v/v) 悬浮于含50%的甘油和15 ppm Kathon的PBS中
Other Notes
亲和凝胶含有亲和纯化的抗HA小鼠单克隆抗体(克隆HA-7)，共价连接于珠状琼脂糖上。 HA标签与人流感病毒血凝素(HA)的98-106氨基酸残基(YPYDVPDYA)相对应。
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
相关内容
默克分享基因组学、克隆学和大量分子生物学技术的发展使研究人员能在众多生物系统中表达异源蛋白。表达重组蛋白的能力为研究人员提供了广泛而强大的下游应用空间，以便进一步开展研究。
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
Peter Moffett
Methods in molecular biology (Clifton, N.J.), 712, 9-20 (2011-03-02)
Plant disease resistance (R) proteins confer protection against specific pathogens or pathogen isolates. R proteins function by recognizing pathogen-encoded avirulence (Avr) proteins and translating this recognition event into an initiation of downstream signaling pathways. Key to understanding this process is
Xudong Guo et al.
Cell stem cell, 22(6), 893-908 (2018-05-15)
Large intergenic non-coding RNAs (lincRNAs) play widespread roles in epigenetic regulation during multiple differentiation processes, but little is known about their mode of action in cardiac differentiation. Here, we identified the key roles of a lincRNA, termed linc1405, in modulating the
The nonreceptor tyrosine kinase c-Abl phosphorylates Runx1 and regulates Runx1-mediated megakaryocyte maturation
Liu H, et al.
Biochimica et biophysica acta. General subjects, 1865(8), 1060-1072 (2018)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| E6779-5X1ML | 04061833604656 |
| E6779-1ML | 04061838099273 |
| E6779-500UL | 04061838248541 |