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Merck
CN

F4135

胎牛血清

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

别名:

HI FBS, HI FCS, HI 血清, FBS, FCS, 血清

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关于此项目

UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
MDL number:
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
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biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

General description

胎牛血清(FBS)一直是一种常用的培养基补充剂,因为它在细胞培养中提供了广泛的功能。FBS提供营养物、生长和附着因子,并有助于增殖。它还保护细胞免于氧化损伤和细胞凋亡。FBS阻断活性氧的生成,否则在无血清培养基(SFM)系统中不会消除这种活性氧。

Application

来自美国的FBS被广泛用于细胞培养应用中。FBS可为培养的细胞提供了多种未确定的生长促进和存活增强因子。
胎牛血清已用于:
  • 作为Dulbecco′s Modified Eagles Media (DME, DMEM)、 Eagle′s Minimum Essential Medium (EMEM)、 Roswell Park Memorial Institute 培养基 (RPMI-1640)、 α-改良 eagle培养基(αMEM) (MEM)和 Leibovitz L-15培养基的成分
  • 作为处理脑干切片的封闭介质的一种成分
  • 用于改善真皮外植体的附着

Preparation Note

在56°C加热30分钟制备。
该产品是在56°C加热30分钟而制备的。将血清加热至56°C 30分钟可灭活补体系统以进行免疫测定。热激活还会使培养物中其他未确定的细胞生长抑制剂失活。

Analysis Note

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次



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存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

监管及禁止进口产品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

商品

钙是一种离子稳定的二价阳离子,在细胞培养基中具有有益和有毒的双重属性,可作为一系列细胞培养基的组成部分。

回顾使用胎牛(或小牛)血清进行细胞培养的基础知识,以及采购和法规如何影响细胞测定所用血清的价格。

查找有关FBS和其他血清产品的常见问题解答(FAQ)以及相关产品的链接。

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Combining probiotic Phaeobacter inhibens DSM17395 and broad-host-range vibriophage KVP40 against fish pathogenic vibrios
Rasmussen BB, et al
Aquaculture (Amsterdam, Netherlands), 513, 734415-734415 (2019)
Ming Guan et al.
American journal of translational research, 11(3), 1343-1358 (2019-04-12)
Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient
Deven Patel et al.
The Journal of biological chemistry, 291(17), 9322-9329 (2016-02-28)
During G1-phase of the cell cycle, normal cells respond first to growth factors that indicate that it is appropriate to divide and then later in G1 to the presence of nutrients that indicate sufficient raw material to generate two daughter



全球贸易项目编号

货号GTIN
F4135-100ML04061838255419
F4135-24X500ML04061833617533
F4135-1L04061837034718
F4135-500ML04061838255426
F4135-6X500ML04061838255433