Anti-Horse Spleen Ferritin antibody produced in rabbit

Ferritin consists of homogenous protein segment called as apoprotein and an iron segment which binds to the apoprotein. It is composed of heavy (H) and light (L) chains.

Ferritin is the storage form of iron in mammals, found mainly in liver, bone marrow and spleen. It is also present in small intestine, placenta, kidney and heart. Horse spleen ferritin is made up of 24 identical subunits. Ferritin of different organs is distinct with different electrophoretic mobility and possible functional differences.
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Purified horse spleen ferritin

Anti-Horse Spleen Ferritin antibody was used for immunocytochemistry of rat brain sections at a working antibody dilution of 1:2000 and in mouse brain sections at 1:1000. The antibody was used for immunoblotting using cell lysates of mouse primary erythroblasts and human ovarian carcinoma cells and human bronchial epithelial cells at a working dilution of 1:1000. The antibody is suitable for immunoelectrophoresis.

Horse Spleen Ferritin antibody has been used:

• immunofluorescence
• in western blotting
• in immunohistochemistry

Ferritin iron is oxidized from Fe(II) to Fe(III) in the presence of the enzyme ferroxidase.

The antibody specifically recognizes ferritin from horse spleen.

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Reconstitute with 0.135 M sodium chloride.

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