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F7425

抗-FLAG® 兔抗

affinity isolated antibody, buffered aqueous solution

别名:

抗-ddddk, 抗-dykddddk

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
MFCD02262912
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, IF, IP, WB CL
Citations:
1955
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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL, immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate, indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK, western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

General description

FLAG表位是一种带有肠激酶切割位点的八氨基酸蛋白。
兔抗-FLAG多克隆亲核抗体抗-FLAG可识别位于FLAG融合蛋白上的FLAG表位。该抗体可与N-末端、N末端-Met及C末端FLAG融合蛋白反应。

Immunogen

FLAG；肽序列DYKDDDDK

Application

采用斑点印迹、免疫印迹、免疫沉淀和免疫细胞化学检测，该抗体可以识别N-末端或C-末端FLAG标记融合蛋白上的FLAG 表位。
请访问我们的 FLAG® 应用门户网站，了解更多产品详情。

Biochem/physiol Actions

FLAG表位在融合蛋白的免疫亲和纯化中具有重要作用。

Physical form

含有1％牛血清白蛋白和15mM 叠氮化钠的0.01M磷酸盐缓冲盐水（pH7.4）中的溶液

Preparation Note

在可承受免疫肽的色谱柱上通过亲和色谱进行纯化。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料
常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
078M4886V
21180417
018M4828V
21171103
097M4882V

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全球贸易项目编号

货号GTIN
F7425-.2MG04061838255501