biological source
bovine fetus
Quality Level
sterility
γ-irradiated, sterile-filtered
product line
FBS Classic
composition
hemoglobin, ≤0.18 mg/mL
origin
Australia origin
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
≤5 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Application
胎牛血清已用于Roswell Park memorial institute培养基-1640 (RPMI-1640),以培养骨髓瘤细胞系,RPMI-8226和U-266。它也被用作Dulbecco改性Eagle培养基(DMEM)的补充物,用于培养人脐静脉内皮细胞(HUVEC)和293 T细胞。
Biochem/physiol Actions
胎牛血清(FBS)由于其高营养成分,是用于补充细胞培养基的最常见的血清。FBS虽然蛋白质含量低,但对促进和维持脊椎动物、哺乳动物和昆虫细胞的生长很有效。它可以作为胚胎干细胞培养的补充物。FBS也可用于制备喂料层和条件介质。
Analysis Note
- 内毒素和血红蛋白测试
Other Notes
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
默克提供血清产品,为您提供高品质澳大利亚胎牛血清和小牛血清现货和解决方案。牛血清由于包含丰富的营养成分,通常被添加到细胞培养液中,用于促进和维持脊椎动物,牛血清的来源地很多,其中澳大利亚来源的牛血清被公认为是品质更优。
回顾使用胎牛(或小牛)血清进行细胞培养的基础知识,以及采购和法规如何影响细胞测定所用血清的价格。
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
相关内容
Instructions
Qingqing Wang et al.
Biochemical and biophysical research communications, 549, 1-7 (2021-03-02)
Ixazomib is a selective, effective, and reversible inhibitor of 20S proteasome and is approved for the treatment of multiple myeloma. Ubiquitin-conjugating enzyme E2 (UBE2K) is involved in the synthesis of K48-linked ubiquitin chains and is the target of certain drugs
Yan Zhang et al.
Artificial cells, nanomedicine, and biotechnology, 48(1), 60-67 (2019-12-20)
Background: Colon cancer is the most frequently lethal cancer in digestive system. Herein, we tested the influences of UNC5B antisense lncRNA 1 (UNC5B-AS1) on colon cancer cell growth and metastasis, along with the regulatory function of UNC5B-AS1 in microRNA-622 (miR-622)
Ludong Zhang et al.
Artificial cells, nanomedicine, and biotechnology, 48(1), 648-655 (2020-02-20)
Recently, the functions of circular RNAs (circRNAs) on cancer initiation and development arouse wide concern. Herein, we tested the influences of circ-ZNF652 on renal carcinoma cell growth and metastasis. Firstly, clinical renal carcinoma tissues and corresponding normal tissues were collected.