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Merck
CN

G0376

β-葡萄糖醛酸酶 来源于牛肝脏

Type B-3, ≥2,000,000 units/g solid

别名:

β-葡萄糖醛酸苷酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
≥2,000,000 units/g solid
Biological source:
bovine liver
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biological source

bovine liver

Quality Level

type

Type B-3

form

solid

specific activity

≥2,000,000 units/g solid

storage temp.

−20°C

Application

β-葡萄糖醛酸酶在GUS测定中可用作报告基因以监测基因的表达。

Biochem/physiol Actions

β-葡萄糖醛酸酶可催化复合碳水化合物的分解。在人体中,它可将结合胆红素转化为非结合的形式,使胆红素利于重吸收。

Other Notes

在37℃,pH 5.0(30分钟检测)条件下,一个Sigma或改良的Fishman单位可每小时从酚酞葡糖苷酸中释放1.0 μg酚酞。


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pictograms

Health hazard

signalword

Danger

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

法规信息

低风险生物材料

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

为了使用β-葡糖醛酸酶优化水解,必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估,例如孵育时间、温度、水解pH、酶源和酶浓度。

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

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