G1544
抗-GFP，N端 兔抗
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
抗-绿色荧光蛋白
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
229
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
绿色荧光蛋白（GFP）生色团含具有Phe64-Ser-Tyr-Gly-Val-Gln69氨基酸序列的六肽片段。GFP结构包含11链β-桶状结构和中心螺旋，对应分子量为26 kDa。
Immunogen
合成多肽对应GFP氨基酸3-17。
Application
兔抗GFP（N末端抗体）抗体用于：
- 蛋白质印迹
- 组织化学和免疫组化
- 免疫沉淀
Biochem/physiol Actions
对于GFP融合蛋白有特异性。
绿色荧光蛋白（GFP）应用于光化学和诊断领域。也广泛应用于蛋白动力学研究。多克隆抗-GFP、兔抗N端抗体可与GFP融合蛋白发生特异性反应。
Physical form
溶于0.01M磷酸盐缓冲盐水，pH 7.4，含有1%牛血清白蛋白以及15 mM叠氮化钠。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
此项目有
A phosphorylation-wide sncRNA screen reveals Protein Functional Effector sncRNAs (pfeRNAs) in human lung somatic cells
Gable T, et al.
Cancer letters, 396(6), 85-93 (2017)
Production and histological application of affinity-purified antibodies to heat-denatured green fluorescent protein
Nakamura KC, et al.
The Journal of Histochemistry and Cytochemistry, 56(7), 647-657 (2008)
Li Yang et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(1), 315-320 (2011-12-21)
Plant microRNAs (miRNAs) typically mediate RNA cleavage, but examples of miRNA-mediated translational repression have also been reported. However, the functional significance of this latter process is unknown. We identified SUO in a screen for Arabidopsis mutations that increase the accumulation
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G1544-100UG | 04061833628270 |