G2150
L -谷氨酰胺 溶液
200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma
别名:
L-Glutamine for cell culture
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关于此项目
化学文摘社编号:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75
grade
Hybri-Max™
Quality Level
sterility
sterile-filtered
assay
≥99% (TLC)
form
solution
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
color
clear colorless
shipped in
dry ice
storage temp.
−20°C
SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Gene Information
rat ... Ggt1(116568)
General description
L-谷氨酰胺是一种必需氨基酸，作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉和冷冻溶液中非常稳定。然而，在液体培养基或储备溶液中，L-谷氨酰胺的降解速度相对更快。为获得最佳细胞性能，通常需要在使用培养基前补充L-谷氨酰胺。
Application
L-谷氨酰胺溶液已用作罗斯威尔公园纪念研究所（RPMI）-1640培养基的补充剂，以培养人白血病单核细胞系用于细胞内分析和脾细胞用于CD4+ T细胞的体外激活。它还用作杂交瘤培养基的补充剂，用于抗原结合和杂交瘤克隆的初级筛选。
Preparation Note
使用细胞培养级水进行制备。
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
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wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
商品
L-谷氨酰胺在杂交瘤细胞和哺乳动物细胞培养中的重要性和应用
Importance and uses of glutamine in hybridoma and mammalian cell culture
Production of human antibodies from transgenic mice.
C Geoffrey Davis et al.
Methods in molecular biology (Clifton, N.J.), 248, 191-200 (2004-02-19)
Abraham L Moure et al.
Journal of medicinal chemistry, 63(9), 4732-4748 (2020-04-11)
Screening of a GSK-proprietary library against intracellular Mycobacterium tuberculosis identified 1, a thioalkylbenzoxazole hit. Biological profiling and mutant analysis revealed that this compound is a prodrug that is bioactivated by the mycobacterial enzyme MymA. A hit-expansion program including design, synthesis
Cosimo Commisso et al.
Nature, 497(7451), 633-637 (2013-05-15)
Macropinocytosis is a highly conserved endocytic process by which extracellular fluid and its contents are internalized into cells through large, heterogeneous vesicles known as macropinosomes. Oncogenic Ras proteins have been shown to stimulate macropinocytosis but the functional contribution of this
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G2150-20ML | 04061838259530 |