G2174
β-葡萄糖醛酸酶 来源于帽贝 (Patella vulgata)
aqueous solution, ≥85,000 units/mL
别名:
β-D-葡萄糖苷酸葡萄糖醛酸水解酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥85,000 units/mL
Biological source:
limpet (Patella vulgata)
biological source
limpet (Patella vulgata)
Quality Level
form
aqueous solution
specific activity
≥85,000 units/mL
secondary activity
≤7,500 units/mL sulfatase
storage temp.
2-8°C
Application
比较不同酶性能的新技术文章
了解更多关于 Sigma R&D 生成的最新应用数据 使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性的影响，导致 6-MAM 转化为吗啡
了解更多关于 Sigma R&D 生成的最新应用数据 使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性的影响，导致 6-MAM 转化为吗啡
据报道，来自欧洲帽贝的酶比来自盖罩大蜗牛、牛肝脏和大肠杆菌的酶能更有效地水解阿片肽-葡糖苷酸
Features and Benefits
Save time with no reconstitution in this convenient liquid format.
Physical form
Aqueous solution in 0.9% NaCl with 0.02% sodium azide as preservative.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
实验方案
为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
-THC solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material
商品
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
相关内容
Instructions
J Combie et al.
Clinical chemistry, 28(1), 83-86 (1982-01-01)
beta-Glucuronidase from Patella vulgata, Helix aspersa, Helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. Factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and pH) were optimized.
T A Jennison et al.
Journal of analytical toxicology, 17(4), 208-210 (1993-07-01)
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose
Tracey B Tucker Zhou et al.
The Journal of biological chemistry, 288(51), 36302-36311 (2013-11-13)
Klotho (KL) is an age-regulating protein named after the Greek goddess who spins the thread of life. Mice deficient in KL are normal throughout development, but rapidly degenerate and display a variety of aging-associated abnormalities that eventually lead to decreased
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G2174-1ML | 04061838087447 |
| G2174-10ML | 04061838087430 |
| G2174-25ML | 04061833628737 |
| G2174-5ML | 04061838087454 |