usage
sufficient for 350 purifications
Quality Level
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
input
plant tissue
test parameters
sample volume: 100 mg
greener alternative category
, Aligned
storage temp.
15-25°C
General description
GenElute植物基因组DNA制备试剂盒可以简单、方便的从各种植物中分离高纯度的基因组DNA。该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、RNase处理，以及诸如苯酚和氯仿等有害化学成分。
首先在液氮中研磨植物组织，在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作，去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤，进一步纯化基因组DNA。
纯化所得的DNA可直接用于PCR、限制性酶切、克隆、测序和Southern印迹等下游应用。
首先在液氮中研磨植物组织，在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作，去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤，进一步纯化基因组DNA。
纯化所得的DNA可直接用于PCR、限制性酶切、克隆、测序和Southern印迹等下游应用。
GenElute植物基因组DNA小量制备试剂盒可以简单、方便的从各种植物中分离高纯度的DNA。GenElute试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、RNase处理，以及诸如苯酚和氯仿等有害化学成分。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法，本产品符合固有安全化学原则(Inherently Safer Chemistry)。
Application
纯化所得的高纯度DNA可直接供以下敏感的下游应用使用：
- PCR
- 限制性内切酶酶切
- 克隆
- Southern印迹
Biochem/physiol Actions
在1小时内，可从至多100 mg新鲜组织或10 mg冻干材料中提取出微克级的DNA。首先在液氮中研磨植物组织，在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作，去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤，进一步纯化基因组DNA。纯化所得DNA长度超过20 kb。
Features and Benefits
- 起始材料：不超过100 mg的植物组织
- 预期得率：至多20 μg
- 洗脱体积：100 - 200 μl
- 所需时间：<40分钟
- 是否需要RNase处理：否
Other Notes
更多信息，请见www.sigma-aldrich.com/genomicdna。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C
supp_hazards
存储类别
8A - Combustible corrosive hazardous materials
wgk
WGK 2
法规信息
新产品
此项目有
实验方案
本方案描述了使用GenElute植物基因组DNA Miniprep试剂盒，从多种植物中分离纯DNA的简单、便捷操作步骤。
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
Minal Mhatre
Methods in molecular biology (Clifton, N.J.), 11013, 435-453 (2012-11-28)
Pineapple (Ananas comosus L., Merr.) is a commercially important crop, grown in the tropical and subtropical regions. However, the crop is faced with postharvest damage and poor varietal and nutritional improvement. Being a vegetatively propagated crop, conventional breeding programs take
Jochen C Reif et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 111(5), 838-845 (2005-07-22)
It has been claimed that the system that delivers the products of plant breeding reduces the diversity of cultivated varieties leading to an increased genetic vulnerability. The main goal of our study was to monitor the temporal trends in genetic
Catherine Ravel et al.
Functional & integrative genomics, 6(4), 310-321 (2006-03-29)
Wheat prolamin-box binding factor (WPBF) was shown to be an activator of Triticum aestivum L. storage protein genes. Three homoeologous genes encoding this transcription factor were isolated from a bacterial artificial chromosome genomic library and sequenced. The genes all have
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G2N350-1KT | 04061835573066 |