Quality Level
technique(s)
affinity chromatography: suitable
matrix active group
phase
swelling
1 g swells to 9-11 mL
bead size
20-80 μm
application(s)
life science and biopharma
compatibility
Cytiva
General description
Sephadex G-50（细颗粒）葡聚糖凝胶是广泛使用的凝胶过滤树脂，设计用于分子量30,000以上生物分子的脱盐和缓冲液置换。因其胶珠较小而具有高效率。不同类型的Sephadex的交联度不同，因此其溶胀程度和分子分离范围也不同。Sephadex G-50属于G型，G型Sephadex的型号包括从小分子G-10到大分子G-75的五种型号。Sephadex G-50分为4种不同粒径，包括粗(Coarse)、中(Medium)、细(Fine)和超细(Superfine)颗粒。
Sephadex® G-50是一种用于亲和层析、蛋白质层析和凝胶过滤层析的凝胶过滤介质。Sephadex 是一种由葡聚糖和环氧氯丙烷交联而成的珠状凝胶。
分馏范围（MW）
球状蛋白质：1,500-30,000
葡聚糖：500-10,000
分馏范围（MW）
球状蛋白质：1,500-30,000
葡聚糖：500-10,000
Application
Sephadex® G-50葡聚糖凝胶适于：
- 去除脂质体悬液中的非包埋羧基荧光素(CF)
- 通过离心式尺寸排阻色谱纯化人MN-14单克隆抗体(mAb)
- 对5和6部分洗脱的重组蛋白进行脱盐
Sephadex® G-50 适用于：
- 低分子量和高分子量分子的分离
- 亲和色谱法、蛋白质色谱法和凝胶过滤色谱法
Features and Benefits
- 一步完成脱盐、除污染和置换新缓冲液。
- 适于通过凝胶过滤纯化DNA，除去小分子。
- 凝胶珠较小，扩散距离更短。
- 经典的凝胶过滤树脂。
- Sephadex 脱盐被认为优于透析，因为它节省时间，稀释系数低，即便使用少量样品也能恢复高活性
Other Notes
G5080-100G′更新后的产品编号为 GE17-0042-01
G5080-500G′更新后的产品编号为 GE17-0042-02
G5080-500G′更新后的产品编号为 GE17-0042-02
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Journal of immunology (Baltimore, Md. : 1950), 151(5), 2877-2886 (1993-09-01)
Bispecific monoclonal antibodies (BsmAb) with specificity for tumor Ag and effector cell trigger molecules have been shown to redirect the cytotoxicity of several peripheral blood mononuclear cell populations against relevant tumor. The BsmAb, 2B1, binds to the extracellular domain of
L Zhang et al.
The EMBO journal, 14(2), 313-320 (1995-01-16)
Heme is a prosthetic group for numerous enzymes, cytochromes and globins, and it binds tightly, sometimes covalently, to these proteins. Interestingly, heme also potentiates binding of the yeast transcriptional activator HAP1 to DNA and inhibits mitochondrial import of the mammalian
Samuel Lopez-Nieves et al.
The New phytologist, 217(2), 896-908 (2017-10-11)
Diverse natural products are synthesized in plants by specialized metabolic enzymes, which are often lineage-specific and derived from gene duplication followed by functional divergence. However, little is known about the contribution of primary metabolism to the evolution of specialized metabolic
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G5080-100G | 04061833632987 |
| G5080-10G | 04061833633007 |
| G5080-50G | 04061833633021 |