G5885
葡萄糖-6-磷酸脱氢酶 来源于肠系膜明串珠菌
lyophilized powder, >= 550 units/mg protein (biuret)
别名:
G-6-P-DH
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
Specific activity:
>= 550 units/mg protein (biuret)
Biological source:
bacterial (Leuconostoc mesenteroides)
biological source
bacterial (Leuconostoc mesenteroides)
Quality Level
type
Type XXIV
form
lyophilized powder
specific activity
>= 550 units/mg protein (biuret)
mol wt
128 kDa
composition
Protein, 15-40% biuret
application(s)
agriculture
foreign activity
6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%, PGI ≤0.01%
storage temp.
2-8°C
General description
葡萄糖-6-磷酸脱氢酶(G-6-PDH)由His-Asp催化二联体构成，并以同二聚体形式存在。结构上，G-6-PDH在辅酶结合结构域内包含罗斯曼折叠构成的二核苷酸结合区域。还具有一个较大的β + α结构域，在374位有一个独特的天冬氨酸残基。
Application
来自肠膜明串珠菌的葡萄糖-6-磷酸脱氢酶已联用己糖激酶进行小鼠肝样品中的葡萄糖测定。
Biochem/physiol Actions
葡萄糖-6-磷酸脱氢酶(G-6-PDH)可使用烟酰胺腺嘌呤二核苷酸磷酸(NADP+)或NAD+作为辅酶，在细菌代谢中具有重要作用。
葡萄糖-6-磷酸脱氢酶(G6PD)催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯，作为磷酸戊糖途径的第一步。
Physical form
含有Ficoll和Tris 缓冲盐的冻干粉
Other Notes
一个单元在NAD 存在下，在pH 7.8，30℃ 下，每分钟将 1.0 μmole的D-葡萄糖-6-磷酸氧化成 6-磷酸-D-葡萄糖酸盐。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
实验方案
在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
V Vought et al.
Biochemistry, 39(49), 15012-15021 (2000-12-07)
The roles of particular amino acids in substrate and coenzyme binding and catalysis of glucose-6-phosphate dehydrogenase of Leuconostoc mesenteroides have been investigated by site-directed mutagenesis, kinetic analysis, and determination of binding constants. The enzyme from this species has functional dual
Enzyme-stabilizing activity of seed trypsin inhibitors during desiccation
Ji-Ming Lam, Keng-Hock Pwee, Wendell Q. Sun, Yii-Leng Chua, Xing-Jun Wang
Plant Science, 142, 209-218 (1999)
J Pozueta-Romero et al.
FEBS letters, 291(2), 233-237 (1991-10-21)
The standardized enzyme coupling method for assaying sucrose synthase activities in the direction of sucrose cleavage was reexamined using enzyme preparations from cultured cells of sycamore (Acer pseudoplatanus L.) and spinach leaves (Spinacea oleracea). Both ATP and Tris, commonly utilized
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G5885-2KU | 04061833638620 |
| G5885-10KU | 04061832729893 |
| G5885-1KU | 04061833638606 |
| G5885-200UN | 04061833638613 |