biological source
Pseudomonas fluorescens
Quality Level
assay
≥30 % (biuret)
form
lyophilized powder
specific activity
≥0.5 units/mg protein
composition
Protein, ≥30% biuret
technique(s)
inhibition assay: suitable
storage temp.
−20°C
General description
研究领域：神经科学
GABase是一种市售酶混合物，由来自于荧光假单胞菌（Pseudomonas fluorescens）的γ-氨基丁酸氨基转移酶（GABGT）和琥珀酸半醛脱氢酶（SSDH）组成。
GABase是一种市售酶混合物，由来自于荧光假单胞菌（Pseudomonas fluorescens）的γ-氨基丁酸氨基转移酶（GABGT）和琥珀酸半醛脱氢酶（SSDH）组成。
Application
来自荧光假单胞菌（Pseudomonas fluorescens）的GABase已被用于GABase测定，以测量GABA（γ-氨基丁酸）的细胞外和细胞内浓度。
Biochem/physiol Actions
GABase 催化中枢神经系统中的γ-氨基丁酸（GABA）转化为琥珀酸。它用于测定各种生物学样品中的GABA水平。对羟基苯甲醛（HBA）是潜在的GABase抑制剂。
Physical form
部分纯化的冻干粉末，含有缓冲盐和稳定剂
Other Notes
一个酶活性单位是指在pH 8.6、25°C下，每分钟将1.0 μmole的γ-氨基丁酸（GABA）转化为琥珀酸半醛、再转化为琥珀酸，并且化学计量还原1.0 μmole的NADP+所需的酶量。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Mingu Gordon Park et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 2016-2024 (2021-09-14)
Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification have not been established. Thus, biochemical and drug
A Vidal-Cros et al.
The Biochemical journal, 229(3), 675-678 (1985-08-01)
L-threo-3-Fluoroglutamate and L-erythro-3-fluoroglutamate were tested with glutamate decarboxylase from Escherichia coli. Both isomers were substrates: the threo isomer was decarboxylated into optically active 4-amino-3-fluorobutyrate, whereas the erythro isomer lost the fluorine atom during the reaction, yielding succinic semialdehyde after hydrolysis
Kimon-Andreas G Karatzas et al.
Applied and environmental microbiology, 76(11), 3529-3537 (2010-04-20)
It is well established that the glutamate decarboxylase (GAD) system is central to the survival of Listeria monocytogenes at low pH, both in acidic foods and within the mammalian stomach. The accepted model proposes that under acidic conditions extracellular glutamate
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G7509-10UN | 04061833642801 |
| G7509-25UN | 04061833492857 |