G7877
葡萄糖-6-磷酸脱氢酶 来源于面包酵母（酿酒酵母）
Type VII, ammonium sulfate suspension, ≥200 units/mg protein
别名:
葡萄糖-6-磷酸脱氢酶(NADP+), 6-磷酸葡糖脱氢酶, G-6-P-DH
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
Specific activity:
≥200 units/mg protein
Biological source:
bakers yeast
biological source
bakers yeast
Quality Level
type
Type VII
form
ammonium sulfate suspension
specific activity
≥200 units/mg protein
mol wt
128 kDa
technique(s)
cell based assay: suitable
solubility
water: soluble
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Gene Information
Saccharomyces cerevisiae ... ZWF1(855480)
General description
葡萄糖6-磷酸脱氢酶（G-6-P-DH）是磷酸戊糖途径第一步的关键调节酶。G-6-P-DH是分子量为128kDa（凝胶过滤）的糖蛋白 。
Application
葡萄糖-6-磷酸脱氢酶已可用于研究去氢表雄酮对TM-3细胞的细胞生长和线粒体功能的影响。葡萄糖-6-磷酸脱氢酶还被用于研究牛卵泡细胞中凋亡和转录本丰度的发生率。葡萄糖-6-磷酸脱氢酶被用于测试玉米胚乳形成中的酮糖还原酶活性。
Biochem/physiol Actions
葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸，作为磷酸戊糖途径的第一步。
Physical form
3.2M (NH4)2SO4 溶液中的晶体悬浮液，pH 7.0
Other Notes
一个酶活性单位是指在pH 7.4、25°C且存在NADP的条件下，每分钟将1.0 μM的 D-葡萄糖6-磷酸氧化成6-磷酸-D-葡糖酸盐所需的酶量。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
实验方案
在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Xuehuai Shen et al.
General and comparative endocrinology, 177(1), 177-186 (2012-04-03)
Dehydroepiandrosterone (DHEA), a major steroid hormone, decreases with age, and this reduction has been shown to be associated with physical health. In the present study, the effect of DHEA on cell growth and mitochondrial function was investigated using TM-3 cells
D Janowski et al.
Theriogenology, 78(3), 656-669 (2012-05-15)
The close contact and interaction between the oocyte and the follicular environment influence the establishment of oocyte developmental competence. Moreover, it is assumed that apoptosis in the follicular cells has a beneficial influence on the developmental competence of oocytes. The
Gang Zhao et al.
Neurochemistry international, 61(2), 146-155 (2012-05-15)
NADPH derived from glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway, has been implicated not only to promote reduced glutathione (GSH) but also enhance oxidative stress in specific cellular conditions. In this study, the effects of G6PD
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G7877-1KU | 04061833643310 |
| G7877-5KU | 04061833643440 |
| G7877-150UN | 04061832730196 |
| G7877-250UN | 04061833643334 |
| G7877-2KU | 04061833643341 |
| G7877-500UN | 04061833643358 |