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Merck
CN

G8295

β-葡萄糖醛酸酶 来源于大肠杆菌

≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

别名:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
≥10,000,000 units/g protein (30 min assay)
Recombinant:
expressed in E. coli overproducing strain
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recombinant

expressed in E. coli overproducing strain

Quality Level

form

lyophilized powder

specific activity

≥10,000,000 units/g protein (30 min assay)

mol wt

~290 kDa

composition

protein, 40-60% biuret

storage temp.

−20°C

Application

比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据,关于使用不同来源的酶优化不同药物类别的水解,并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。

Analysis Note

含有缓冲盐和稳定剂。

Other Notes

一个Sigma或改良的Fishman单元将在37℃,pH6.8(30分钟测定)下每小时从酚酞葡糖苷酸中释放1.0 μg酚酞。


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pictograms

Health hazard

signalword

Danger

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

法规信息

常规特殊物品

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

为了使用β-葡糖醛酸酶优化水解,必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估,例如孵育时间、温度、水解pH、酶源和酶浓度。

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

商品

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.


Enzymes
Methods of Enzymatic Analysis, 2, 460-461 (1974)
Ling Xiao et al.
RSC advances, 10(39), 22966-22971 (2020-06-16)
UDP-glucuronosyltransferases (UGTs) and β-glucuronidase (GUS) catalyze entirely distinct metabolism reactions. UGTs are responsible for the glucuronidation of a variety of drugs, endogenous and environmental chemicals, whereas GUS hydrolyzes glucuronides and liberates the parent substrates. Information on the overlap of ligand
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine.
Tsujikawa, K., et al.
Health Laboratory Science, 50, 286-289 (2004)



全球贸易项目编号

货号GTIN
G8295-25KU04061833218792
G8295-2MU04061833218808
G8295-500KU04061833218815