G8529
葡萄糖-6-磷酸脱氢酶 来源于肠系膜明串珠菌
recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)
别名:
G-6-P-DH
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
Specific activity:
≥550 units/mg protein (biuret)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
form
lyophilized powder
specific activity
≥550 units/mg protein (biuret)
composition
Protein, 10-40% biuret
application(s)
agriculture
foreign activity
creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
storage temp.
2-8°C
Application
肠系膜明串珠菌来源葡萄糖 6-磷酸脱氢酶已用于：
- PGI 的偶联分光光度测定法（F6P-G6P 反应）
- 使用烟草叶研究淀粉时程
- 测定北美黑橡(Quercus velutina Lam. saplings)根和茎样品中的葡萄糖含量
- 使用野生型和转基因拟南芥整株幼苗的淀粉样本测定葡萄糖
Biochem/physiol Actions
6-磷酸葡萄糖脱氢酶 (G6PD) 在所有组织中均有表达，并产生还原形式的烟酰胺腺嘌呤二核苷酸磷酸 (NADPH)，有助于细胞避免氧化压力。G6PD不足是一种 X 连锁的遗传性基因缺陷，由G6PD基因常见突变引起。
葡萄糖-6-磷酸脱氢酶(G6PD)催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯，作为磷酸戊糖途径的第一步。
Physical form
含有Ficoll和Tris 缓冲盐的冻干粉
Other Notes
在 NAD 存在下，在pH 7.8，30°C 下，1 个单位将每分钟氧化 1.0 μmol D-6-磷酸葡萄糖至 6-磷酸-D-葡萄糖酸盐。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
实验方案
在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
Alyssa L Preiser et al.
Frontiers in plant science, 11, 580726-580726 (2020-12-29)
Phosphoglucoisomerase (PGI) isomerizes fructose 6-phosphate (F6P) and glucose 6-phosphate (G6P) in starch and sucrose biosynthesis. Both plastidic and cytosolic isoforms are found in plant leaves. Using recombinant enzymes and isolated chloroplasts, we have characterized the plastidic and cytosolic isoforms of
Ching Man Wai et al.
PLoS genetics, 15(6), e1008209-e1008209 (2019-06-15)
Plants with facultative crassulacean acid metabolism (CAM) maximize performance through utilizing C3 or C4 photosynthesis under ideal conditions while temporally switching to CAM under water stress (drought). While genome-scale analyses of constitutive CAM plants suggest that time of day networks
Thomas Beneyton et al.
Nature communications, 9(1), 2391-2391 (2018-06-21)
Self-sustained metabolic pathways in microcompartments are the corner-stone for living systems. From a technological viewpoint, such pathways are a mandatory prerequisite for the reliable design of artificial cells functioning out-of-equilibrium. Here we develop a microfluidic platform for the miniaturization and
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G8529-2KU | 04061833644096 |
| G8529-10KU | 04061832730318 |
| G8529-1KU | 04061833644089 |
| G8529-200UN | 04061832730332 |