biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
liquid
technique(s)
dot blot: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
聚组氨酸(小鼠IgG2a)单克隆抗体可识别天然或变性的还原型合成聚组氨酸和聚组氨酸标签融合蛋白。 该抗体优先识别N-末端标签融合蛋白,并且与原核表达载体pET、pRSET和pTrc表达的融合蛋白反应。
Immunogen
重组聚组氨酸标签融合蛋白。
Application
该抗体适用于免疫印迹、斑点杂交印迹、ELISA和免疫细胞化学分析。小鼠聚组氨酸单克隆抗体可用于蛋白质免疫印迹分析。
Biochem/physiol Actions
IgG2a(免疫球蛋白G 2a)基因主动参与刺激小鼠的自身免疫。该基因在病毒感染中高表达。
该抗体可识别合成聚组氨酸,以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。
Physical form
以含有15mM叠氮化钠的腹水形式提供。
Other Notes
用于免疫印迹的建议稀释度为1:3000
Legal Information
本产品受专利DE 19507166以及独家授权给 QIAGEN GmbH(Qiagen Strasse 1, D-40724 Hilden, Germany)的国外同等专利的保护。
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存储类别
10 - Combustible liquids
法规信息
常规特殊物品
此项目有
Elise Pinta et al.
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Prokunina-Olsson L, et al.
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Garcia-Regalado A, et al.
Molecular Biology of the Cell, 19(10), 4188-4200 (2008)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| H1029-.2ML | 04061838446121 |
| H1029-.5ML | 04061833663790 |
| H1029-100UL | 04061838446138 |