Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit

Histone Deacetylase (HDACs) have been described as six or seven different groups in mammals. HDAC1, HDAC2 and HDAC3 are like yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are like yeast Hda1 (Histone Deacetylase 1) protein.

synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively).

Anti-Histone Deacetylase 2 (HDAC2) antibody has been used:

• in immunoblotting and dot blot
• in immunoprecipitation
• in immunohistochemistry
• in enzyme linked immunosorbent assay (ELISA)
• in western blotting
• in chromatin immunoprecipitation(ChiP)

Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin.

Histone acetylation is a dynamic process whose levels are determined by the net activities of Histone acetyl transferase (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosome condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

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