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H3631

透明质酸酶 来源于牛睾丸

Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid

别名:

玻璃糖醛酸酶, 玻璃酸酶

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关于此项目

化学文摘社编号:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
EC 号:
3.2.1.35 (BRENDA, IUBMB)
Specific activity:
3,000-15,000 units/mg solid
Biological source:
bovine testis
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biological source

bovine testis

Quality Level

200

conjugate

conjugate (Glucosaminoglycan)

type

Type VI-S

form

lyophilized powder

specific activity

3,000-15,000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

Q5E985, Q8SQG8

relevant disease(s)

cancer

storage temp.

−20°C

Gene Information

cow ... HYAL1(515397), HYAL2(281838)

Biochem/physiol Actions

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。

Preparation Note

色谱纯化产品

Other Notes

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。
更多关于复杂碳水化合物分析酶的信息，请见www.sigma-aldrich.com/enzymeexplorer

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLBZ0772
SLBZ3166
SLBV2044
SLBQ5073V
SLBJ9451V

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实验方案

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

商品

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Tongcui Ma et al.
eLife, 11 (2022-07-06)
High-parameter single-cell phenotyping has enabled in-depth classification and interrogation of immune cells, but to date has not allowed for glycan characterization. Here, we develop CyTOF-Lec as an approach to simultaneously characterize many protein and glycan features of human immune cells
Dong-Ru Ho et al.
International journal of urology : official journal of the Japanese Urological Association, 18(7), 525-531 (2011-05-25)
To investigate how hyaluronic acid (HA) affects nerve growth factor (NGF) production and bladder overactivity in a cyclophosphamide (CYP)-induced cystitis rat model. Female Sprague-Dawley rats received three intermittent intraperitoneal injections of CYP (75 mg/kg) or saline. Before or after CYP injection
Tongcui Ma et al.
eLife, 9 (2020-05-27)
The female reproductive tract (FRT) is the most common site of infection during HIV transmission to women, but viral remodeling complicates characterization of cells targeted for infection. Here, we report extensive phenotypic analyses of HIV-infected endometrial cells by CyTOF, and
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全球贸易项目编号

货号GTIN
H3631-15KU04061833793619
H3631-30KU04061833793657
H3631-3KU04061833793671