H4272
透明质酸酶 来源于牛睾丸
Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid
别名:
玻璃糖醛酸酶, 玻璃酸酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.75
EC Number:
253-464-3
MDL number:
Specific activity:
750-3000 units/mg solid
sterility
aseptically processed
Quality Level
type
Type IV-S
form
powder
specific activity
750-3000 units/mg solid
mol wt
~55 kDa (four subunits of 14 kDa each)
composition
Protein, 80-105% biuret
technique(s)
cell culture | embryo: suitable
storage temp.
−20°C
Application
透明质酸酶是一种来自牛睾丸的酶。准备用于体外培养的卵子时，可利用这种酶去除小鼠胚胎分离物或新排卵中的卵丘物质。
促性腺激素用于使20只雌性小鼠超排卵。然后小鼠进行交配，获得并计数着床前期胚胎。然后胚胎使用透明质酸酶处理，知道其中所有积云物质都从胚胎里去除掉。胚胎被培育，每天查看，记录胚胎发育进展。
促性腺激素用于使20只雌性小鼠超排卵。然后小鼠进行交配，获得并计数着床前期胚胎。然后胚胎使用透明质酸酶处理，知道其中所有积云物质都从胚胎里去除掉。胚胎被培育，每天查看，记录胚胎发育进展。
Biochem/physiol Actions
这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。
Preparation Note
用3 ml M2培养基（货号M 7167或M 5910）或去离子水复溶1瓶透明质酸酶，获得10 mg/ml的储备溶液。用M2培养基稀释储备溶液，至约300 μg/ml最终工作浓度。
Other Notes
在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| H4272-30MG | 04061835568659 |