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Merck
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HSRT100

增强型禽类HS RT-PCR试剂盒

Flexible kit for one-step or two-step RT-PCR

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关于此项目

NACRES:
NA.55
UNSPSC Code:
41106303
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Quality Level

usage

sufficient for 100 reactions

feature

dNTPs included, hotstart

technique(s)

RT-PCR: suitable, cDNA synthesis: suitable

color

colorless

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

General description

提供1步法和2步法RT-PCR反应。

1步法: 同一管中的eAMV RT逆转录酶和AccuTaq LA DNA聚合酶相继发挥作用,首先生成cDNA,紧接着进行PCR扩增反应。 用于灵敏、快速的RNA分析。

2步法: 每次反应都经过单独优化,可在高保真前提下获得更高产率。适合需要更多扩增的实验,或高产率比便利性更加重要的实验。

Application

增强型禽类HS RT-PCR试剂盒用于合成反转录酶PCR (RT-PCR) 分析的第一条cDNA链。

Features and Benefits

  • 该反转录酶可制备更长的转录本,生成长达14.1kb的cDNA。
  • 低丰度mRNA检测灵敏度更高。eAMV RT能够成功转录其他反转录酶无法检测到的微量RNA。
  • 针对复杂的二级结构的RNA,拥有超强的转录能力。
  • JumpStart AccuTaq LA DNA聚合酶有利于提高灵敏度、特异性与得率。

Other Notes

Reverse Transcriptase PCR (RT-PCR)是一种强大的基因表达研究工具。Enhanced Avian HS RT-PCR试剂盒应用了一款加强型禽成髓细胞瘤病毒逆转录酶(eAMV-RT),相比标准型AMV-RT和MMLV-RT具有更佳的反应性能。eAMV RT是一种效率极强的酶,在较高温度下(65 °C)仍能够打开复杂RNA的二级结构成功介导转录反应,是以总RNA或poly(A)+ RNA为模板制备高质量全长cDNA(长达14.1kb)的最佳酶蛋白。同时本品也附赠JumpStartTM AccuTaq LA DNA聚合酶混合物,可减少非特异性扩增,增加反应特异性与灵敏度。这两种酶的组合为用户提供了一个高品质的反应系统,能够同时兼容一步法或两步法的RT-PCR方案。

Legal Information

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
eAMV is a trademark of Sigma-Aldrich Co. LLC


试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书 & 价格

  • Random nonamers 100 μL
    化学品安全说明书

  • Anchored oligo(dT)23 primers 100 μL
    化学品安全说明书

  • 10x reaction buffers 10x AccuTaq buffer
    化学品安全说明书

  • 10 mM dNTP mix 10 x PCR buffer
    化学品安全说明书

  • Nuclease-free water 4 x 1.5
    化学品安全说明书

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

signalword

Danger

wgk

WGK 3

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1

法规信息

常规特殊物品
低风险生物材料

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

商品

分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战,例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

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用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具,用于基于 mRNA 的疫苗和疗法开发。

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全球贸易项目编号

货号GTIN
HSRT100-1KT04061833826546