产品名称
Iscove 改良杜氏培养基, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
components
phenol red: yes
glucose: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Iscove改良杜氏培养基是杜氏改良Eagle培养基(DME)的改良版本，含有硒、额外的氨基酸和维生素、丙酮酸钠、4-(2-羟乙基)-1-哌嗪乙磺酸(HEPES)缓冲液、以及硝酸钾（代替硝酸铁）。据报道，Iscove培养基可支持鼠B淋巴细胞、骨髓造血组织、脂多糖刺激的B细胞、T淋巴细胞和多种杂交细胞的生长。
Application
Iscove改良杜氏培养基已用于KG1a细胞（人白血病细胞株）和中国仓鼠卵细胞(CHO)DG44细胞株的培养。
据报道，其可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞的生长。
Preparation Note
补充 3.024 g/L 碳酸氢钠。
配制成每升培养基中含 17.7 克粉末。
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存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Lucía Flores-Peredo et al.
Molecular carcinogenesis, 56(2), 735-750 (2016-07-20)
The Sarco/Endoplasmic Reticulum Ca
Hale M Tasyurek et al.
Human gene therapy, 29(7), 802-815 (2018-02-08)
Postprandial glucose-induced insulin secretion from the islets of Langerhans is facilitated by glucagon-like peptide-1 (GLP-1)-a metabolic hormone with insulinotropic properties. Among the variety of effects it mediates, GLP-1 induces delta cell secretion of somatostatin, inhibits alpha cell release of glucagon
Ryan S Pawell et al.
Biomicrofluidics, 7(5), 56501-56501 (2014-01-10)
Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell separation and enrichment. Currently, there are no cost-effective manufacturing methods for this promising microfluidic technology. In this fabrication paper, however, we develop a
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| I7633-10X1L | 04061833858332 |
| I7633-10L | 04061833858325 |
| I7633-50K | 04061834180449 |
| I7633-50L | 04061833858349 |