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Merck
CN

I8896

IGEPAL® CA-630

Molecular Biology

别名:

辛基苯氧基聚(乙烯氧基)乙醇,支链, 聚氧乙烯辛烷基苯酚醚,分支, 辛基苯基-聚乙烯二醇

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关于此项目

线性分子式:
(C2H4O)nC14H22O
化学文摘社编号:
UNSPSC Code:
12352104
NACRES:
NA.31
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grade

Molecular Biology

description

non-ionic

form

liquid

technique(s)

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

viscosity

~250-350 cP(25 °C)

mp

6 °C

solubility

water: soluble at 20 °C

density

1.060 g/cm3

HLB

13

foreign activity

DNase and RNase, none detected

SMILES string

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

General description

IGEPAL® CA-630是一种非离子型、非变性去污剂,适用于膜蛋白复合物的溶解、分离和纯化。它与Nonidet P-40在化学上无法区分。

Application

IGEPAL® CA-630已用于:
  • 在小鼠肌肉匀浆液的蛋白质印迹分析(WB)中,作为匀浆缓冲液的中性去垢剂
  • 在E8.5-E10.5胚胎的lacZ表达中,用于X-gal染色
  • 加入细胞裂解(CL)缓冲液中,制备RT-qPCR即用型细胞裂解液

Analysis Note

在化学性质方面,不能与已经退市的 Nonidet P-40 区分开。

Other Notes

非离子型、非变性洗涤剂

Legal Information

IGEPAL is a registered trademark of Solvay

Disclaimer

在贮存过程中可能产生混浊或沉淀物。 可在加热至时40°C变为澄清液体。


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signalword

Danger

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

相关内容

利用亲和力、GST pull-down、TAP 和共免疫沉淀方法,通过Pull-Down研究体外蛋白质互作。

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.


Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained



全球贸易项目编号

货号GTIN
I8896-50ML04061838256003
I8896-100ML04061838255990