conjugate
agarose conjugate
Quality Level
form
saline suspension
extent of labeling
~6 mg per mL
matrix
4% agarose
matrix activation
cyanogen bromide
matrix spacer
1 atom
particle size
45-165 μm
capacity
≥0.5 mg/mL binding capacity (ovomucoid)
storage temp.
2-8°C
Application
用于纯化含有(D-N-乙酰氨基葡萄糖)2或N-乙酰神经氨酸的糖蛋白。
Biochem/physiol Actions
WGA 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。
Physical form
含15 mM叠氮化钠的0.9% NaCl中的混悬剂。
Preparation Note
由亲和纯化凝集素制备偶联物。
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存储类别
10 - Combustible liquids
法规信息
动植物源性产品
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实验方案
This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.
商品
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
相关内容
Glycobiology and Glycoproteomics Brochure
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The normal cellular prion protein (PrP) is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein. However, in pancreatic ductal adenocarcinoma cell lines, such as BxPC-3, PrP exists as a pro-PrP retaining its glycosylphosphatidylinositol (GPI) peptide signaling sequence. Here, we report the identification
D L Christie et al.
The Biochemical journal, 270(1), 57-61 (1990-08-15)
Chromaffin-granule membranes were separated into insoluble and soluble fractions after extraction with lithium di-iodosalicylate (LDIS). These fractions were characterized by one- and two-dimensional gel electrophoresis, and glycoproteins were detected after electroblotting with peroxidase-labelled concanavalin A and wheat-germ agglutinin (WGA). The
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L1394-1ML | 04061833949399 |
| L1394-5ML | 04061838163912 |
| L1394-10ML | 04061833949382 |