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Merck
CN

L1668

来自 菜豆的凝集素 (红芸豆)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

别名:

PHA, 植物血球凝集素(菜豆)

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)
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Quality Level

product line

BioReagent

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

color

white to off-white

solubility

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

storage temp.

2-8°C

Application

来自菜豆(Phaseolus vulgaris) (红芸豆)的凝集素已被用于:
  • 刺激分化簇(CD4+)T细胞
  • 激活原发性外周血淋巴细胞
  • 刺激外周血单核细胞

Biochem/physiol Actions

PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。

Packaging

根据凝集素的含量进行出售

Analysis Note

其效力和溶解度以mg凝集素/ml表示


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

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全球贸易项目编号

货号GTIN
L1668-5MG04061835557455