conjugate
agarose conjugate
Quality Level
form
saline suspension
extent of labeling
5-10 mg per mL
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
capacity
≥0.1 mg/mL binding capacity (ovomucoid)
storage temp.
2-8°C
General description
凝集素是糖类结合蛋白,广泛存在于真菌、植物和动物中。凝集素的二级结构富含 β-链,表面具有糖类结合位点。
Application
用于纯化含有(D-N-乙酰氨基葡萄糖)2或N-乙酰神经氨酸的糖蛋白。
Biochem/physiol Actions
凝集素可用于鉴定、成像和靶向糖缀合物。与寡糖、糖蛋白和糖脂发生特异性相互作用。因此,凝集素用于微阵列测定,可实现有效的糖组谱分析。在植物和真菌内部,凝集素可抵御病原体或寄生物(feeder)侵袭。还参与宿主识别和组织粘附。
WGA 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。
Physical form
悬浮于含15 mM叠氮化钠的1.0 M NaCl溶液中
Preparation Note
由亲和纯化凝集素制备偶联物。
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存储类别
10 - Combustible liquids
wgk
WGK 3
ppe
Eyeshields, Gloves
法规信息
动植物源性产品
此项目有
实验方案
本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。
该页面展示了如何使用Cytiva的Tricorn™或XK色谱柱进行亲和层析色谱柱填充和制备。
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
相关内容
Glycobiology and Glycoproteomics Brochure
Binding sugars: from natural lectins to synthetic receptors and engineered neolectins
Arnaud J, et al.
Chemical Society Reviews, 42(11), 4798-4813 (2013)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L1882-1ML | 04061838163929 |
| L1882-5ML | 04061838163936 |