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Merck
CN

L2380

来自西非单叶豆加纳籽)的凝集素

lyophilized powder

别名:

西非单叶豆 凝集素, BS-I

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
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form

lyophilized powder

Quality Level

potency

<20 μg per mL agglutination activity (using human blood group B erythrocytes)

composition

Protein, ≥90% biuret

impurities

salt, free

storage temp.

2-8°C

Biochem/physiol Actions

BS-I对末端α-D-半乳糖残基具有主要亲和力,对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。

Preparation Note

Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).

Analysis Note

凝集活性以 μg/mL表示,并且使用磷酸盐缓冲盐水(pH6.8)对1mg/mL溶液进行连续稀释液来测定,对于每种凝集素,所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后,对2%合适的红细胞悬浮液进行凝集所需的最低浓度。

Other Notes

BS-I是一种四聚体凝集素,由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素:BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的,其对末端α-D-半乳糖残基具有独特的亲和力,而BSI-A4 具有血型A特异性,其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

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历史批次信息供参考:

分析证书(COA)

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全球贸易项目编号

货号GTIN
L2380-5MG04061833263112
L2380-1MG04061833950029