L2524
溶壁酶 来源于藤黄节杆菌
lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret
别名:
细菌溶壁酶, 裂解酶
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
Specific activity:
≥2,000 units/mg protein
Biological source:
bacterial (Arthrobacter luteus)
biological source
bacterial (Arthrobacter luteus)
Quality Level
form
lyophilized powder
specific activity
≥2,000 units/mg protein
composition
Protein, ≥20% biuret
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Application
藤黄节杆菌(Arthrobacter luteus)溶壁酶用于：
- 制备原生质球
- 作为消化液组分，用于孵育酵母细胞消化细胞壁
- 酶解菌丝体沉淀以制备原生质体
藤黄节杆菌（Arthrobacter luteus ）裂解酶已用于裂解真菌细胞壁，分离DNA。
Biochem/physiol Actions
酵母细胞很难被破坏，因为其细胞壁可能形成胶囊或耐药孢子。可通过裂解酶（如溶壁酶）诱导部分原生质球形成，从酵母中提取DNA。随后再裂解原生质球以释放DNA。溶壁酶更易消化酵母菌细胞壁，并从真菌中产生原生质球进行转化。它含有β-(1→3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。溶壁酶适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌( Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。
溶壁酶(Lyticase)常用于真菌研究，特别是用于PCR法（聚合酶链式反应）物种鉴定。可断链糖基之间的β(1→3)和β(1→4)键。溶菌酶是巨噬细胞介导宿主反应的指示剂，与白细胞死亡有关，具有高周转率。多种慢性炎症、炎症性肠病、血液病和肾病中都发现血清溶菌酶浓度提高。鸡蛋白中的C型溶菌酶常用作蛋白结构和功能的研究模型。胞壁质酶主要对革兰氏阳性菌具有杀菌活性。手册所述凝胶过滤色谱法测定分子量是现有发表方法的改良版。该试剂盒中的蛋白标准品可能适合其他色谱方法，如高效液相色谱(HPLC)。但是，某些缓冲体系可能会影响白蛋白和碳酸酐酶的洗脱体积。该试剂盒中的蛋白分子量范围为29 kDa至699 kDa。
溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。
Physical form
部分纯化的冻干粉末，含有磷酸钾缓冲盐和稳定剂
Other Notes
一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA800 。
仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。
Still not finding the right product?
Explore all of our products under 溶壁酶 来源于藤黄节杆菌
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
实验方案
本操作程序可用于使用面包酵母作为底物测定溶壁酶的活性。
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
相关内容
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
Hagen Frickmann et al.
BMC microbiology, 19(1), 75-75 (2019-04-10)
The potential of next-generation sequencing (NGS) for hypothesis-free pathogen diagnosis from (poly-)microbially contaminated, formalin-fixed, paraffin embedded tissue samples from patients with invasive fungal infections and amebiasis was investigated. Samples from patients with chromoblastomycosis (n = 3), coccidioidomycosis (n = 2), histoplasmosis (n = 4), histoplasmosis or
Daniel Zenklusen et al.
Nature structural & molecular biology, 15(12), 1263-1271 (2008-11-18)
Proper execution of transcriptional programs is a key requirement of gene expression regulation, demanding accurate control of timing and amplitude. How precisely the transcription machinery fulfills this task is not known. Using an in situ hybridization approach that detects single
Joana Segura et al.
Nature communications, 9(1), 3989-3989 (2018-09-30)
The interplay between chromatin structure and DNA topology is a fundamental, yet elusive, regulator of genome activities. A paradigmatic case is the "linking number paradox" of nucleosomal DNA, which refers to the incongruence between the near two left-handed superhelical turns
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L2524-10KU | 04061835573363 |
| L2524-200KU | 04061835573370 |
| L2524-25KU | 04061835573387 |
| L2524-50KU | 04061835573394 |