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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-954-0
MDL number:
Specific activity:
≥35,000 units/mg protein (E1%/282)
Biological source:
chicken egg white
biological source
chicken egg white
Quality Level
type
Grade VI
form
lyophilized powder
specific activity
≥35,000 units/mg protein (E1%/282)
mol wt
~14.3 kDa
composition
Protein, ≥80%
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
application(s)
cell analysis
storage temp.
−20°C
General description
溶菌酶,又称为胞壁质酶,是存在于诸如白细胞之类的吞噬细胞中的细菌裂解酶。溶菌酶的命名是因为它能溶解或水解细菌细胞壁中N-乙酰氨基葡萄糖(NAG)和N-乙酰胞壁酸(NAM)之间的β-1-4-糖苷键。该蛋白质存在于动物、植物、细菌和病毒中。还存在于中性粒细胞颗粒、巨噬细胞中,以及血清、唾液、奶、蜂蜜和鸡蛋白中。溶菌酶分为不同类型,包括c型(鸡型)、g型(鹅型)和i型(无脊椎动物型)。
Application
溶菌酶(氯化物形式,来自鸡蛋白)用于:
- 作为蛋白混合物组分,研究在死端过滤中酵母细胞对蛋白混合物引起的膜污染的影响
- 组成浸没系统,研究其对沙门菌污染肉鸡的抑菌或杀菌作用
- 在黏度试验中测定溶菌酶活性
Biochem/physiol Actions
溶菌酶是巨噬细胞介导宿主反应的指示剂,与白细胞死亡有关,具有高周转率。多种慢性炎症、炎症性肠病、血液病和肾病中都发现血清溶菌酶浓度提高。鸡蛋白中的C型溶菌酶常用作蛋白结构和功能的研究模型。胞壁质酶主要对革兰氏阳性菌具有杀菌活性。
Physical form
含氯化钠和乙酸钠的冻干粉。
Preparation Note
3×结晶
Other Notes
酶用缓冲液悬浮后,在pH 6.2、25 °C下,1单位每分钟可释放0.6 μg溶壁微球菌(浊度法检测,600nm)。
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
A Sobrino-López et al.
Journal of dairy science, 91(5), 1759-1768 (2008-04-19)
High-intensity pulsed electric field (HIPEF) is a nonthermal treatment studied for its wide antimicrobial spectrum on liquid food, including milk and dairy products. Moreover, the antimicrobial effect of HIPEF may be enhanced by combining HIPEF with other treatments as hurdles.
R S Pappas et al.
Journal of analytical toxicology, 32(4), 281-291 (2008-04-24)
The extent to which smokeless tobacco endangers human health is an ongoing subject of debate. Studies have shown that smokeless tobacco products contain high levels of biologically available nicotine and tobacco-specific nitrosamines. Toxic metals in smokeless tobacco products have been
Sven O Dahms et al.
Acta crystallographica. Section D, Biological crystallography, 69(Pt 2), 284-297 (2013-02-07)
Heavy-atom clusters (HA clusters) containing a large number of specifically arranged electron-dense scatterers are especially useful for experimental phase determination of large complex structures, weakly diffracting crystals or structures with large unit cells. Often, the determination of the exact orientation
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L2879-25G | 04061833950456 |
| L2879-1G | 04061833950449 |
| L2879-5G | 04061833950470 |