L4025
溶壁酶 来源于藤黄节杆菌
lyophilized powder, ≥200 units/mg solid
别名:
细菌溶壁酶, 裂解酶
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关于此项目
Specific activity:
≥200 units/mg solid
Biological source:
bacterial (Arthrobacter luteus)
biological source
bacterial (Arthrobacter luteus)
Quality Level
form
lyophilized powder
specific activity
≥200 units/mg solid
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Application
藤黄节杆菌（Arthrobacter luteus）裂解酶已用于：
- 作为球状体形成形缓冲液成分，制备酵母核提取物
- 消化样品制剂，用酵母进行β-葡聚糖酶促测定
- 裂解细胞以便RNA分离
藤黄节杆菌(Arthrobacter luteus)溶壁酶可用于重悬细胞以提取高分子量DNA用于PCR（聚合酶链式反应）指纹分析。
Biochem/physiol Actions
溶壁酶是一种裂解酶，用于通过诱导部分原生质球形成，从酵母细胞中提取DNA。随后再裂解原生质球以释放DNA。酵母细胞的细胞壁可能形成荚膜或耐药孢子，很难破坏，因此溶壁酶成为消化酵母细胞壁的首选方案。溶壁酶含有β-(1→;3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。它适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌( Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。
溶壁酶(Lyticase)常用于真菌研究，特别是用于PCR法（聚合酶链式反应）物种鉴定。可切开糖基之间的β (1→3)和β (1→4)键。
溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。
Other Notes
一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA800 。
仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
实验方案
本操作程序可用于使用面包酵母作为底物测定溶壁酶的活性。
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L4025-100KU | 04061835573486 |
| L4025-250KU | 04061833957226 |
| L4025-25KU | 04061835573493 |
| L4025-1MU | 04061833957219 |
| L4025-50KU | 04061835573509 |