L5283
白血病抑制因子 人
≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
别名:
LIF, rLIF
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关于此项目
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
buffered aqueous solution (pH 7.4)
产品名称
白血病抑制因子 人, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture
recombinant
expressed in E. coli
Quality Level
assay
≥95% (SDS-PAGE)
form
buffered aqueous solution (pH 7.4)
potency
0.1-1.5 ng/mL ED50/EC50
quality
endotoxin tested
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
Biochem/physiol Actions
白血病抑制因子（LIF）是一种多效性糖蛋白，最初被发现在诱导分化成巨噬细胞时，可抑制小鼠骨髓白血病细胞系M1的增殖。LIF的其他活性后来被陆续发现，人们可能了解其各种同义名，包括DIF、D因子、DIA、DRF、CNDF、HILDA、HSF-III和MLPLI。人LIF通过包含190kDa LIF结合α-链（130kDa，小鼠）和130kDa信号转导β-链（gp130）的受体发挥其作用，其与CNTF、OSM、L-6和IL-11公用同一受体。因此，它是细胞因子受体超家族gp130家族的成员。LIF受体已在几种细胞中得到鉴定，包括单核细胞、肝脏、胎盘和胚胎干细胞。天然LIF是高度糖基化的，研究显示根据来源不同，其分子量为32kDa至62kDa，但是没有糖基化似乎不影响其生物活性。LIF由单个基因编码，在人或小鼠中其分泌为含有180个氨基酸的单链糖蛋白，并具有保守的二硫键。人和小鼠LIF共享78％的序列同源性。人LIF可激活小鼠细胞，但小鼠LIF不能激活人体细胞。
白血病抑制因子（LIF）是一种多效性糖蛋白，最初被发现在诱导分化成巨噬细胞时，可抑制小鼠骨髓白血病细胞系M1的增殖。人和小鼠LIF共享78％的序列同源性。人LIF可激活小鼠细胞，但小鼠LIF不能激活人体细胞。
Physical form
溶于含有0.02％吐温® 20的磷酸盐缓冲盐水中的溶液（经 0.2 μm过滤）。
Analysis Note
使用人白血病细胞系TF-1在培养物中对人LIF的增殖活性进行测定。
Legal Information
TWEEN is a registered trademark of Croda International PLC
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
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商品
Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).
Naive pluripotent stem cells cultured in vitro using specialized media and inhibitors mimic "ground-state" cells from blastocysts.
Magda Tomala et al.
Protein expression and purification, 73(1), 51-57 (2010-04-13)
Leukemia inhibitory factor (LIF) is a polyfunctional cytokine with numerous regulatory effects in vivo and in vitro. In stem cell cultures it is the essential media supplement for the maintenance of pluripotency of embryonic and induced pluripotent stem cells. With
N M Gough et al.
Proceedings of the National Academy of Sciences of the United States of America, 85(8), 2623-2627 (1988-04-01)
A human homologue of the recently cloned murine leukemia-inhibitory factor (LIF) gene was isolated from a genomic library by using the murine cDNA as a hybridization probe. The nucleotide sequence of the human gene indicated that human LIF has 78%
Borhane Annabi et al.
Journal of neuroinflammation, 6, 8-8 (2009-03-11)
The CD133(+) stem cell population in recurrent gliomas is associated with clinical features such as therapy resistance, blood-brain barrier disruption and, hence, tumor infiltration. Screening of a large panel of glioma samples increasing histological grade demonstrated frequencies of CD133(+) cells
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L5283-10UG | 04061833958001 |
| L5283-5X10UG | 04061833386262 |