L6010
α-乳清蛋白 来源于牛奶
Type III, calcium depleted, ≥85% (PAGE), lyophilized powder
别名:
Bos d 4, Lactose synthase B protein, alpha-lactalbumin
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关于此项目
Biological source:
bovine milk
Assay:
≥85% (PAGE)
Form:
lyophilized powder
Technique(s):
indirect ELISA: suitable
Impurities:
cation traces, tested
Concentration:
≥85 % protein
biological source
bovine milk
Quality Level
type
Type III
assay
≥85% (PAGE)
form
lyophilized powder
quality
calcium depleted
mol wt
14,178 Da by calculation
concentration
≥85 % protein
technique(s)
indirect ELISA: suitable
impurities
cation traces, tested
solubility
H2O: soluble 10 mg/mL, clear to slightly hazy, colorless to faintly yellow
cation traces
Ca: ≤0.3 mol/mol
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... LALBA(281894)
General description
可能含有微量的(NH4)2SO4和磷酸钠L6010
Application
α-牛乳乳清蛋白已用于：
- 间接酶联免疫吸附测定（ELISA）和竞争性ELISA方法
- Hsp90和HSJ1b结合分析
- 抑制溶菌酶CAP-RAST测定体系
Biochem/physiol Actions
α-乳清蛋白是人乳中的主要蛋白。它由含有8个半胱氨酸（可形成二硫键）的单个多肽链组成。α-乳清蛋白可结合包括钙在内的几种金属离子，这被认为有助于从还原的变性形式α-乳清蛋白中再生天然形式的α-乳清蛋白。α-乳清蛋白还具有独特的锌结合位点，其被认为在乳糖合酶复合物的结合中起作用。其成熟蛋白由123个氨基酸残基（14kD）组成，具有1.7埃分辨率的三维结构，包含四个α-螺旋和三链反平行β-折叠。
其可通过改变半乳糖基转移酶的底物特异性以增加乳糖形成的速率；半乳糖基转移酶和 α-乳白蛋白的复合物称为乳糖合酶。
其可通过改变半乳糖基转移酶的底物特异性以增加乳糖形成的速率；半乳糖基转移酶和α-乳白蛋白的复合物称为乳糖合酶。Asp87 或 Asp88 对 Ala 的定点诱变完全消除了强钙结合亲和力，并将乳糖合酶的刺激降低到最大速率的 <3.5%。
Analysis Note
可能含微量 (NH4)2SO4 和磷酸钠。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
B M Fu et al.
The American journal of physiology, 274(6 Pt 2), H2062-H2073 (1998-06-25)
We previously proposed a two-pathway model for solute and water transport across vascular endothelium (Fu, B. M., R. Tsay, F. E. Curry, and S. Weinbaum. J. Biomech. Eng. 116: 502-513, 1994) that hypothesized the existence of a continuous slit 2
Bingmei M Fu et al.
American journal of physiology. Heart and circulatory physiology, 284(6), H2124-H2135 (2003-02-01)
To investigate the ultrastructural mechanisms of acute microvessel hyperpermeability by vascular endothelial growth factor (VEGF), we combined a mathematical model (J Biomech Eng 116: 502-513, 1994) with experimental data of the effect of VEGF on microvessel hydraulic conductivity (L(p)) and
Shang Shen et al.
Experimental physiology, 95(2), 369-379 (2009-11-03)
To investigate whether vascular endothelial growth factor (VEGF) enhances cancer cell adhesion to normal microvessels, we used in vivo video microscopy to measure adhesion rates of MDA-MB-435s human breast cancer cells and ErbB2-transformed mouse mammary carcinomas in the postcapillary venules
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L6010-500MG | 04061833263501 |
| L6010-100MG | 04061833958568 |
| L6010-1G | 04061833958575 |