L7773
Lysozyme 来源于鸡蛋白
aseptically filled
别名:
粘肽N-乙酰胞壁质水解酶, 胞壁质酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
235-747-3
MDL number:
sterility
aseptically filled
Quality Level
form
powder
enzyme activity
≥40000 units/mg protein
mol wt
single-chain 14.3 kDa
UniProt accession no.
storage temp.
−20°C
Gene Information
chicken ... LYZ(396218)
Application
酶可分解细菌的细胞壁；用于制备原生质体。
Biochem/physiol Actions
溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。 革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感，因为它们有外膜并且肽聚糖的比例较低。然而，这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。
该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。
该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。
溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。
Preparation Note
使用L6876制备
Other Notes
酶用缓冲液悬浮后，在pH 6.2、25 °C下，1单位每分钟可释放0.6 μg溶壁微球菌（浊度法检测，600nm）。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
Sven O Dahms et al.
Acta crystallographica. Section D, Biological crystallography, 69(Pt 2), 284-297 (2013-02-07)
Heavy-atom clusters (HA clusters) containing a large number of specifically arranged electron-dense scatterers are especially useful for experimental phase determination of large complex structures, weakly diffracting crystals or structures with large unit cells. Often, the determination of the exact orientation
Agnieszka Zdybicka-Barabas et al.
Biochimica et biophysica acta, 1828(6), 1449-1456 (2013-02-20)
Insect immune response relies on the humoral and cellular mechanisms of innate immunity. The key factors are the antimicrobial polypeptides that act in concert against invading pathogens. Several such components, e.g. apolipophorin III (apoLp-III), lysozyme, and anionic peptide 2, are
Zhe Wang et al.
The journal of physical chemistry. B, 117(4), 1186-1195 (2013-01-11)
High-resolution inelastic X-ray scattering was used to investigate the collective vibrational excitations in hydrated lysozyme powders as a function of hydration level and temperature. It is found that the samples with strong enzymatic function are "soft", in the sense that
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L7773-50MG | 04061833965696 |